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目的 :探讨核黄素和抗坏血酸在体内对脱氧雪腐镰刀菌烯醇 (DON)诱发小鼠胸腺细胞凋亡的阻断作用。方法 :采用动物实验 ,应用DNA琼脂糖凝胶电泳和流式细胞术检测方法分析核黄素和抗坏血酸预处理对DON诱导小鼠胸腺细胞凋亡和增殖抑制的影响。结果 :FCM检测结果表明 ,4mg/kgDON可诱导小鼠胸腺细胞凋亡 ,其凋亡率为 1 3 73 %± 1 53 %。核黄素 (1 2 5 - 1 0mg/kg)和抗坏血酸 (2 5 - 1 0 0mg/kg)预处理组小鼠胸腺细胞凋亡率均明显低于DON组 (P <0 0 5) ,但不同剂量核黄素和抗坏血酸预处理组间凋亡率无明显差异。DNA琼脂糖凝胶电泳结果亦证实 ,DON处理组细胞出现明显的凋亡特有的“梯状条带” ,而核黄素和抗坏血酸预处理各组及生理盐水对照组细胞均未出现“梯状条带”。给予核黄素和抗坏血酸预处理的各DON处理组细胞增殖指数与DON处理组细胞增殖指数之间无明显差异。结论 :核黄素和抗坏血酸均可一定程度抑制DON体内对小鼠胸腺细胞的致凋亡作用 ,但对DON抑制胸腺细胞增殖的作用无明显影响
Objective: To investigate the inhibitory effect of riboflavin and ascorbic acid on the apoptosis of mouse thymocytes induced by deoxynivalenol (DON) in vivo. Methods: The effects of riboflavin and ascorbic acid preconditioning on the apoptosis and proliferation of mouse thymocytes induced by DON were analyzed by using animal experiments and DNA agarose gel electrophoresis and flow cytometry. Results: The result of FCM showed that 4mg / kg DON could induce thymocyte apoptosis in mice with the apoptosis rate of 13 73% ± 153%. The apoptotic rates of thymocytes in the preconditioned group treated with riboflavin (125-500 mg / kg) and ascorbic acid (25-1000 mg / kg) were significantly lower than those in the DON group (P <0.05) There was no significant difference in apoptotic rates between different doses of riboflavin and ascorbic acid pretreatment groups. DNA agarose gel electrophoresis results also confirmed that DON-treated cells showed obvious apoptosis-specific “ladder-like”, and riboflavin and ascorbic acid pretreatment groups and saline control group cells did not appear “ladder-like ”. There was no significant difference between the cell proliferation index of DON treated with riboflavin and ascorbic acid pretreatment and the cell proliferation index of DON treated group. CONCLUSION: Both riboflavin and ascorbic acid can inhibit the apoptosis of mouse thymocytes induced by DON to a certain extent, but have no obvious effect on the inhibition of thymocyte proliferation by DON