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目的了解山东地区不同人群中SEN病毒(SENV)感染的状况与其致病性。方法应用逆转录聚合酶链反应法(RT-PCR)检测了2545份不同人群和肝病患者血清中SENVDNA,并对17例不明原因ALT增高,单独SENVDNA阳性的非甲—非戊型肝炎患者行肝活检。结果SENVDNA总阳性检出率为20.87%(53/254),其中非甲—非戊型肝炎血清检出率最高为59.57%(28/47),明显高于健康献血员3.23%(1/31)、非肝病患者5.45%(3/55)、输血后丙型肝炎42.86%(12/48)、血液透析患者15.38%(2/13)和慢性乙肝患者8.75%(7/80),SENV在各种人群中存在着显著性差异(P<0.01)。SENV感染其主要病理学改变为汇管区炎症,淋巴细胞集聚,碎小坏死,小叶内病变较轻,呈散在和点状坏死,而桥样坏死,脂肪性变较少。结论山东地区不同人群及肝病患者中存在着较高SENV感染率;SENV感染可能具有嗜肝性;而且可能与肝功损害有关,是引起非甲—非戊型肝炎重要病原,输血后丙肝和有血液透析史患者SENV感染率较高,提示对献血员还应严格筛选。
Objective To understand the status and pathogenicity of SENV infection among different populations in Shandong Province. Methods The serum levels of SENVDNA in 2545 different population and liver disease patients were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). ALT was increased in 17 patients with unexplained causes. The patients with non-A-hepatitis E positive SENVDNA alone had liver cirrhosis Biopsy. Results The positive rate of SENVDNA was 20.87% (53/254). The highest detection rate of non-A-non-E hepatitis was 59.57% (28/47), which was significantly higher than that of healthy donors (3.23%, 1/31 , 5.45% (3/55) in non-liver disease, 42.86% (12/48) in blood transfusions, 15.38% (2/13) in hemodialysis patients and 8.75% (7/80) in patients with chronic hepatitis B There were significant differences in various populations (P <0.01). SENV infection of the main pathological changes for the portal area inflammation, lymphocyte aggregation, broken small necrosis, lobular lesions were mild, scattered and punctate necrosis, and bridge-like necrosis, less fatty changes. Conclusions There is a high prevalence of SENV infection in different populations and patients with liver diseases in Shandong Province. SENV infection may have hepatic necrosis and may be related to liver damage, which is an important pathogen causing non-A-hepatitis E infection. Hemodialysis patients with higher prevalence of SENV, suggesting that blood donors should also be strictly screened.