论文部分内容阅读
为探讨角膜碱烧伤后反复上皮糜烂的免疫学因素,制作 SD 大鼠角膜中央(B1组)及角膜缘(B2组)碱烧伤模型,分4个时间点取眼球;制作正常及碱烧伤 SD 大鼠角膜、角膜缘及结膜铺片标本,用单克隆抗体CD3(T 细胞)、CD4(辅助性 T 细胞)、CD8(抑制性 T 细胞)、ED1~+(巨噬细胞、单核细胞、树突状细胞)及 OX6(MHC-Ⅱ类抗原)行免疫组化染色。结果表明,伤后5天左右,B1组及 B2组多数实验动物出现持续或反复角膜糜烂。多为有新生血管者,持续约4周。免疫组化染色结果显示,正常鼠仅在角膜缘和结膜见少量上述阳性细胞;伤后第3天左右,实验鼠角膜、角膜缘及结膜可见大量上述阳性细胞,阳性细胞出现时间与角膜糜烂持续时间相一致,B1组阳性细胞数量相对少,但持续时间与 B2组相近。结论:角膜碱烧伤后出现反复上皮糜烂与新生血管有关,与角膜缘是否损伤无关;多种细胞参与的免疫反应可能为其发病机制之一。
To investigate the immunological factors of repeated epithelial erosion after alkali burns in cornea, the model of alkali burn in central cornea (group B1) and corneal limb (group B2) of SD rats was made, and the eyeballs were made at 4 time points. SD and normal alkali burn The corneal, limbal and conjunctival popliteal specimens were stained with monoclonal antibodies CD3 (T cells), CD4 (T helper cells), CD8 (suppressor T cells), ED1 ~ + (macrophages, monocytes, DCs) and OX6 (MHC-Ⅱ antigen) immunohistochemical staining. The results showed that the majority of experimental animals in groups B1 and B2 had persistent or recurrent corneal erosion about 5 days after injury. Mostly have new blood vessels who lasted about 4 weeks. Immunohistochemical staining showed that only a small amount of these positive cells were seen in the corneal limbal and conjunctiva in normal rats. On the 3rd day after injury, a large amount of above-mentioned positive cells were observed in the cornea, limbus and conjunctiva of rats, and the appearance time of positive cells and corneal erosion continued At the same time, the number of positive cells in group B1 was relatively small, but the duration was similar to group B2. CONCLUSION: Repeated epithelial erosion after corneal alkali burn is related to neovascularization and has nothing to do with corneal limbal injury. A variety of cellular immune responses may be one of its pathogenesis.