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目的 :了解布比卡因中毒时诱发心律失常在中枢的调控部位 ,为进一步研究局部麻醉药中毒对心血管调控中枢的变化机理打下基础.方法 :用SD大鼠 12只 ,建立布比卡因中毒性心律失常模型.应用免疫组织化学方法 ,光镜观察Fos蛋白阳性神经元在中枢神经的分布.结果 :布比卡因中毒在外侧缰核Fos阳性神经元的数量为 76± 13 ,外侧臂旁核 2 6± 8,孤束核 2 5± 6,三叉神经尾侧亚核 16± 4 ;而正常对照组这些核团内无Fos阳性神经元的表达.两组比较差别非常显著.结论 :布比卡因中毒性心律失常与中枢神经系统外侧缰核 ,外侧臂旁核 ,三叉神经尾侧亚核和孤束核的机能状态有联系 ,其机理尚待进一步研究“,”Objective: To understand the regulation and control of the position in the central nervous system when toxic arrhythmia is induced by bupivacaine. Methods: Using Fos oncoprotein antibody immunohitochemical technique (ABC method), the distribution of c fos oncogene expressin in the brian was observed with microscope. Results: There were four Fos like immunoreactive neuron groups in relation to bupivacaine arrhythmia. The numbers in lateral habenular nucleus (LHb) were 76±13; nucleus of the solitary tract (Sol) 25±6, spinal trigerminal nucleus, caudal (Sp5C) 16±4, lateral parabrachial nucleus (LPB) 26±8. There were no Fos like immunoreactive neurons in the nucleus of control group. Conclusion: The results indicate that the arrhythmia induced by bupivacaine is related to LHB, Sol, Sp5C and LPB.