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考察了紫草提取物在体外和体内的抗氧化活性。以合成天然抗氧化剂二叔丁基对甲酚(BHT)和天然抗氧化剂α-生育酚(α-tocopherol)为对照,用OSI方法测定了紫草石油醚提取物和氯仿提取物在猪油中的抗氧化作用,并首次考察了二者对小鼠血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平的影响。结果表明,添加质量分数为0.1%的紫草石油醚和氯仿提取物,可分别使猪油的氧化诱导期由4.5 h延长至52.4 h和52.2 h。小鼠灌服紫草石油醚和氯仿提取物21 d后,其血清中SOD活性均显著高于正常组(P<0.05或P<0.01),而MDA含量显著低于正常组(P<0.05或P<0.01),表现出体内抗氧化活性,证明紫草石油醚和氯仿提取物具有较强抗氧化活性,可显著抑制猪油氧化,并可明显提高小鼠体内SOD的活性,降低MDA含量。该研究内容的新颖性,已为教育部科技查新工作站(G05)2008年2月28日出具的的第DHU2008-019号《科技查新报告》所证实。
The antioxidant activity of comfrey extract in vitro and in vivo was investigated. Using the natural antioxidants di-tert-butyl-p-cresol (BHT) and the natural antioxidant α-tocopherol as controls, the petroleum ether extract and chloroform extract of lazate were determined by the OSI method in lard And the effect of the two on the activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) in serum of mice for the first time. The results showed that the addition of petroleum jelly and chloroform extract with mass fraction of 0.1% could prolong the oxidation induction period of lard from 4.5 h to 52.4 h and 52.2 h, respectively. The serum SOD activity of mice was significantly higher than that of normal group (P <0.05 or P <0.01) and MDA content was significantly lower than that of normal group (P <0.05 or P <0.01) P <0.01), and showed antioxidant activity in vivo. It was proved that Lithospermum oil ether and chloroform extract had strong antioxidant activity, significantly inhibited the oxidation of lard oil, and significantly increased SOD activity and MDA content in mice. The novelty of this research has been confirmed by the Science and Technology Review No. DHU2008-019 issued on February 28, 2008 by the Science and Technology Check Station (G05) of the Ministry of Education.