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目的:研究胶质母细胞瘤(GBM)组织照射60 Gy后基因表达谱中免疫相关基因表达差异的变化及意义。方法:应用含13 929条人类全长基因cDNA表达谱芯片BioStarH-141s(2004)对两例胶质母细胞瘤组织进行放疗前及放疗60 Gy后检测,并分析它们之间的基因表达差异。结果:人脑胶质母细胞瘤放射治疗60 Gy后与放射治疗前比较,表达差异基因中改变最明显的功能群是免疫系统相关的基因,如IGLV1-44、SLPI、CXCL14等上调。细胞增殖、细胞调亡、细胞周期、DNA修复系统也有部分基因发生明显变化,如MLL5上调、POLR2B下调等。结论:提示对胶质母细胞瘤组织照射60 Gy后基因表达谱改变的研究可以更好地阐明放射敏感性差异机理,为放疗前或放疗早期寻找到预测放射敏感性分子标志提供理论依据。
OBJECTIVE: To study the changes and significance of the expression of immune-related genes in gene expression profiles after gioblastoma (GBM) irradiated 60 Gy. METHODS: Two cases of glioblastoma tissue were treated with radiotherapy and radiotherapy for 60 Gy, using 13 929 human cDNA microarray BioStarH-141s (2004). The difference of gene expression between them was analyzed. Results: Compared with that before radiotherapy, the most obvious functional group in the differentially expressed genes of glioblastoma 60 Gy after radiotherapy was immune system related genes such as IGLV1-44, SLPI, CXCL14 and so on. Some genes such as MLL5 up-regulation and POLR2B down-regulation have also been observed in cell proliferation, apoptosis, cell cycle and DNA repair system. Conclusion: The study of the change of gene expression profile in glioblastoma tissues after 60 Gy irradiation can clarify the difference mechanism of radiosensitivity, and provide a theoretical basis for finding the molecular markers for predicting radiosensitivity before radiotherapy or early stage of radiotherapy.