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目的 探讨转移生长因子 (TGF) β、碱性成纤维细胞生长因子 (bFGF)对人成骨细胞血小板衍生生长因子 (PDGF) BmRNA表达的影响及其意义。方法 体外分离培养人成骨细胞。在体外培养的成骨细胞中分别加入 10、2 0、40、80、16 0 μg/L梯度浓度的PDGF BB培养细胞 2 4h ,以摄取3H TdR为细胞增殖指标检测细胞增殖状况。以 4μg /L的TGF β和 10 μg/L的bFGF培养细胞 2 4h ,寡核苷酸探针检测细胞PDGF BmRNA的表达。结果 10～ 16 0 μg/L的PDGF BB可促进成骨细胞增殖 (P <0 .0 5 )。在普通培养条件下 ,细胞不表达PDGF BmRNA ;当培养体系中加入TGF β和bFGF ,可见PDGF BmRNA的表达。结论 PDGF B基因的表达可能是骨组织生长的储备因素 ,TGF β和bFGF可诱导成骨细胞表达PDGF BB
Objective To investigate the effect and significance of transforming growth factor (TGF) β and basic fibroblast growth factor (bFGF) on the expression of platelet-derived growth factor (PDGF) B mRNA in human osteoblasts. Methods Human osteoblasts were isolated and cultured in vitro. The osteoblasts cultured in vitro were treated with PDGF BB at a concentration of 10, 20, 40, 80, and 160 μg / L for 24 hours, respectively. 3H-TdR was used as a marker of cell proliferation to detect cell proliferation. The cells were cultured with 4μg / L TGFβ and 10μg / L bFGF for 24 hours. The expression of PDGF B mRNA was detected by oligonucleotide probe. Results PDGF BB (10 ~ 160 μg / L) promoted osteoblast proliferation (P <0.05). Under ordinary culture conditions, the cells do not express PDGF B mRNA; when TGF β and bFGF are added to the culture system, the expression of PDGF B mRNA can be seen. Conclusion The expression of PDGF B gene may be the reserve factor for the growth of bone tissue. TGF β and bFGF can induce osteoblast to express PDGF BB