组织培养技术发展很快,但上皮细胞培养仍存在较大困难,一是在培养过程中成纤维细胞生长较旺盛,难以形成纯上皮细胞群;二是上皮细胞难以在体外长期生存。因此,纯化和延长生存期是上皮细胞培养的关键。本方法在原有实验条件的基础上,对实验方法进行了一些改进,获得了较纯化的原代人胚肝上皮细胞,且生长的细胞数量基本上能满足进行体外转化等实验需要。取胎龄4～6月左右水囊引产的胎儿肝脏,在冷L15液中洗去血液,用锋利刀片将胚肝切成1～2mm~3大小的 Tissue culture technology is developing rapidly, but there are still major difficulties in epithelial cell culture. First, fibroblasts grow more vigorously during culture and it is difficult to form pure epithelial cell populations. Second, it is difficult for epithelial cells to survive long-term in vitro. Therefore, purification and prolongation of survival is the key to epithelial cell culture. Based on the original experimental conditions, this method has made some improvements on the experimental methods, and obtained more purified primary human embryonic liver epithelial cells, and the number of cells grown can basically meet the experimental needs for in vitro transformation. Fetal age of 4 to 6 months to take fetus induced abortion of the liver in cold L15 liquid wash away the blood, with a sharp blade of embryo liver cut into 1 ~ 2mm ~ 3 size