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目的：建立体外大量扩增树突状细胞（ Ｄ Ｃ）的方法，并对 Ｄ Ｃ进行形态学和免疫学性质鉴定。方法：制备小鼠骨髓细胞，用免疫磁珠方法去除其中的淋巴细胞、单核巨噬细胞及粒细胞，将阴性选择到的细胞用小鼠重组 Ｇ Ｍ Ｃ Ｓ Ｆ培养，８ ｄ 后收集悬浮细胞。将收集到的细胞进行免疫表型鉴定和超微结构观察，以及 Ｄ Ｃ特异性抗原 Ｄ Ｅ Ｃ２０５ 的免疫胶体金标记。结果：可从每只小鼠的股骨骨髓中扩增到（２～５）×１０６ 个 Ｄ Ｃ， 细胞纯度达８０％ 以上。细胞表达 Ｄ Ｃ的特异性抗原 Ｄ Ｅ Ｃ２０５，具有 Ｄ Ｃ的典型形态及表型。结论：成功地建立了体外大量扩增骨髓 Ｄ Ｃ的方法，为深入研究其免疫学功能打下基础。
OBJECTIVE: To establish a method to amplify dendritic cells (DCs) in vitro in large quantities and to identify the morphological and immunological properties of Dc. Methods: The mouse bone marrow cells were prepared, and the immunomagnetic beads were used to remove the lymphocytes, monocytes and granulocytes. Negatively selected cells were cultured in mouse recombinant G M-CSF and collected 8 days later Suspension cells. The collected cells were identified by immunophenotyping and ultrastructure observation, and immunogold labeling of D C-specific antigen D E C205. RESULTS: Amplification of (2 ~ 5) × 106 D C from femur bone marrow of each mouse resulted in cell purity of more than 80%. The cells express D C specific antigen D E C205, which has the typical morphology and phenotype of D C. CONCLUSION: The method of massively expanding bone marrow D C in vitro has been successfully established, laying a foundation for further study of its immunological function.