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目的:探讨MUC15和PI3K/Akt的表达与胃癌临床病理特征和预后的关系。方法:采用组织芯片和免疫组织化学法检测144例胃癌组织及对应癌旁组织中MUC15、Akt的表达。结果:MUC15在胃癌中阳性表达率为79.8%,高于癌旁组织中的阳性表达率22.2%(P<0.01);Akt在胃癌中阳性表达率为80.6%,高于癌旁组织的阳性表达率16.7%(P<0.01)。MUC15和Akt表达均与胃癌分化程度、浸润深度、有无淋巴结转移和TNM分期相关(P<0.05);且胃癌组织中MUC15表达和Akt表达呈正相关(P=0.001)。单因素生存分析显示,MUC15和Akt高表达均与生存时间呈负相关(P<0.05)。Cox多元回归分析提示,MUC15和Akt同时阳性的胃癌患者预后更差(HR=3.115,P<0.05)。结论:MUC15可能通过PI3K/Akt细胞信号转导通路参与胃癌的发生、发展、浸润转移,MUC15结合Akt是胃癌预后强有力的预测因子。
Objective: To investigate the relationship between the expression of MUC15, PI3K / Akt and the clinicopathological features and prognosis of gastric cancer. Methods: The expression of MUC15 and Akt in 144 cases of gastric cancer tissues and corresponding paracancerous tissues was detected by tissue microarray and immunohistochemistry. Results: The positive rate of MUC15 in gastric cancer was 79.8%, which was higher than that in paracancerous tissues (22.2%, P <0.01). The positive rate of Akt in gastric cancer was 80.6%, which was higher than that in paracancerous tissues The rate was 16.7% (P <0.01). The expression of MUC15 and Akt were correlated with the degree of differentiation, depth of invasion, lymph node metastasis and TNM stage (P <0.05). The expression of MUC15 was positively correlated with Akt in gastric cancer (P = 0.001). Univariate survival analysis showed that MUC15 and Akt overexpression had a negative correlation with survival time (P <0.05). Cox multivariate regression analysis showed that patients with MUC15 and Akt positive gastric cancer had worse prognosis (HR = 3.115, P <0.05). Conclusions: MUC15 may be involved in the occurrence, development, infiltration and metastasis of gastric cancer through the PI3K / Akt cell signaling pathway. MUC15 combined with Akt is a powerful predictor of gastric cancer prognosis.