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细胞膜Na+/H+逆转运蛋白SOS1在植物耐盐过程中起重要作用.本研究将盐生植物海马齿中的细胞膜Na+/H+逆转运蛋白基因SpSOS1构建到植物双元表达载体pCAMBIA 1304上,采用农杆菌介导的方法将SpSOS1基因转入到拟南芥中,在含有50 μg/mL潮霉素B的MS培养基上筛选转基因后代,结合目标基因和标记基因的PCR验证,获得了超表达的转基因拟南芥.利用T3代转基因幼苗进行耐盐性分析.研究表明,转SpSOS1基因的拟南芥在50 mmol/L NaCl的培养基中发芽率在80％以上,明显高于野生型拟南芥的45％.转SpSOS1基因拟南芥幼苗的耐盐性明显提高,在100 mmol/L NaC1条件下能正常生长,具有较高的生物量、根长和侧根数,而野生型拟南芥在50 mmol/L NaC1的胁迫下明显萎蔫、失绿、甚至死亡.说明过表达SpSOS1基因显著提高了拟南芥的耐盐性.“,”Plasma membrane Na+/H+ antiporter SOS1 (salt overly sensitive) plays significant role in plant salt tolerance.In this study,a Na+/H+ antiporter SOS1 gene from Sesuvium portulacastrum was constructed into the pCAMBIA1304 binary vector,and the SpSOS1 gene was transformed into Arabidopsis by using the agrobacterium-mediated transformation.The SpSOS1 over-expressing transgenic plants were obtained by screened transgenic plants on MS medium containing 50 μg/mL Hygromycin B and confirmed by the PCR verification of target gene and marker gene.T3 transgenic seedlings were used to analyze the salt tolerance.The results showed that Arabidopsis over-expressing SpSOS1 germination rate was up to 80％ under 50 mmol/L NaCl treatment,which obviously overtopped than wild type plants with the 45 percent.The salt tolerance of SpSOS1 transgenic plants significantly improved,and grew up normally under 100 mmol/L NaC1 treatment with the high biomass,root length and lateral root number.However the wild type plants were wilted,degraded green even died.This study showed that over-expressing the Sesuvium portulacastrum SpSOS1 gene could enhance salt tolerance of transgenic Arab idop s is.