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目的调查参与某次氦氧饱和实验的潜水作业人群皮肤粘膜日常携带铜绿假单胞菌及其毒力情况。方法用生化鉴定法对细菌进行分类;稀释平板法分析分离菌株生长特征;分光光度法测定绿脓菌素含量;动物染毒法观察分离菌毒力。结果 27名潜水员中有6名被检出携带铜绿假单胞菌,检出率为22.22%,其生长曲线与对照组铜绿假单胞菌标准株PAO1的生长曲线差异无统计学意义(P>0.05);被检出的6株铜绿假单胞菌中有4株绿脓菌素试验为阳性;分离菌株均能造成小鼠致死性感染,绿脓菌素测定为阴性的分离菌株致死率低于绿脓菌素测定为阳性的分离菌株(P<0.05)。结论在潜水人群中,铜绿假单胞菌广泛存在,在高气压潜水作业过程中极易发生急性感染,应采取必要防控措施。
Objective To investigate the daily carriage of Pseudomonas aeruginosa and its virulence in the skin and mucous membranes of diving workers involved in a helium-oxygen saturation experiment. Methods The biochemical identification method was used to classify bacteria. The growth characteristics of isolates were analyzed by dilution plate method. The pyocyanin content was determined by spectrophotometry. The toxicity of isolates was observed by animal exposure method. Results Six of the 27 divers were detected with Pseudomonas aeruginosa (Pseudomonas aeruginosa), the detection rate was 22.22%. There was no significant difference between the growth curve and the growth curve of PAO1 of Pseudomonas aeruginosa standard strain (P> 0.05). Four strains of Pseudomonas aeruginosa were tested positive for pyocyanin. All isolates could cause lethal infection in mice and low lethality for pyocyanin-negative isolates Isolates were positive for pyocyanin (P <0.05). Conclusions Among the diving population, Pseudomonas aeruginosa is widespread and acute infection can easily occur during the operation of high pressure diving. Necessary prevention and control measures should be taken.