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目的探讨成年大鼠纹状体内生长抑素(SOM)阳性神经元的形态分布。方法选取6只SD大鼠,应用免疫细胞化学染色ABC法观察脑纹状体SOM神经元的分布特征。结果在大鼠纹状体内SOM免疫反应阳性神经元呈棕黄色,为强阳性或中等阳性染色,免疫反应阳性物质主要存在于胞质和突起。SOM阳性神经元在纹状体呈散在分布,胞体中等大小[(111.81±21.42)μm2],多为卵圆形、椭圆型或多边形。背侧[(113.52±22.36)μm2]SOM阳性神经元胞体面积大于腹侧[(110.14±20.59)μm2],外侧[(126.23±23.45)μm2]大于内侧[(97.26±23.63)μm2]。腹内侧区SOM阳性细胞数量百分比最多[(28.34±3.20)%],其次是背内侧区[(27.64±3.52)%]和腹外侧区[(24.98±3.17)%],背外侧区最少[(19.04±3.12)%]。结论 SOM神经元在纹状体结构各区分布的大小及数量不完全相同。
Objective To investigate the morphological changes of somatostatin (SOM) positive neurons in adult rat striatum. Methods Six Sprague-Dawley rats were selected and the distribution of SOM neurons in the striatum was observed by immunocytochemical ABC staining. Results The SOM immunoreactive neurons in the striatum of rats showed brownish yellow, strongly or moderately positive staining. The positive immunoreactive substances mainly existed in the cytoplasm and protrusions. SOM positive neurons scattered in the striatum, the size of the cell body is medium size [(111.81 ± 21.42) μm2], mostly oval, oval or polygonal. (113.52 ± 22.36) μm2] in the dorsal side of the SOM positive neurons was larger than that in the ventral [(110.14 ± 20.59) μm2] and [(126.23 ± 23.45) μm2] sides of the dorsal side [(97.26 ± 23.63) μm2]. The number of SOM positive cells in the ventral medial area was the highest (28.34 ± 3.20%), followed by the dorsal medial area (27.64 ± 3.52%) and the ventral lateral area (24.98 ± 3.17%), while the dorsolateral medulla [ 19.04 ± 3.12)%]. Conclusion The size and number of SOM neurons in different regions of striatum are not exactly the same.