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目的检测P33ING1及PINCH蛋白在脑胶质瘤中的表达,探讨其在胶质瘤发生、发展中的作用。方法选取行脑胶质瘤手术切除的新鲜胶质瘤标本50例,应用S-P免疫组织化学方法检测P33ING1和PINCH在瘤组织及正常组织中的表达。结果50例胶质瘤标本中P33ING1阳性表达率为90%(45/50),正常组织中全部表达阳性(100%);随着肿瘤级别的增高,P33ING1表达水平下降,2者呈负相关(P<0.01)。PINCH蛋白的阳性表达率为76%(38/50),正常组织中有3例表达,阳性率为37.5%(3/8);随着肿瘤级别的增高,PINCH表达水平越高,2者呈正相关(P<0.01)。P33ING1与PINCH在胶质瘤中的蛋白表达水平(LI)呈明显的负相关(r=-0.5422,P<0.01)。结论抑癌基因P33ING1的表达对脑胶质瘤细胞增殖起抑制作用,而作为信号连接蛋白PINCH的表达则对胶质瘤的侵袭与转移起促进作用。P33ING1与PINCH的表达存在相关性,是影响脑胶质瘤发生发展的重要因素。
Objective To detect the expression of P33ING1 and PINCH protein in glioma and to explore the role of P33ING1 and PINCH in the development and progression of glioma. Methods Fifty fresh glioma specimens were selected for resection of glioma. The expression of P33ING1 and PINCH in tumor tissue and normal tissue was detected by S-P immunohistochemistry. Results The positive expression rate of P33ING1 was 90% (45/50) in 50 cases of gliomas, and all of them were positive (100%) in normal tissues. The expression of P33ING1 was decreased with the increase of tumor grade, but negative correlated P <0.01). The positive expression rate of PINCH protein was 76% (38/50) in 3 cases and 37.5% (3/8) in normal tissues. The higher the level of PINCH, the higher the PINCH expression Related (P <0.01). P33ING1 and PINCH in glioma protein expression level (LI) showed a significant negative correlation (r = -0.5422, P <0.01). Conclusion The expression of tumor suppressor gene P33ING1 can inhibit the proliferation of glioma cells. However, the expression of PINCH, as a signal pathway protein, promotes the invasion and metastasis of glioma. P33ING1 and PINCH expression is related, is an important factor affecting the occurrence and development of glioma.