Objective: To study the drug property differences of Shexiang (Moschus) and Bingpian (Beolum Synthcticum) with biothermodynamics, and to verify the objectivity and authenticity of the drug property. Methods: The growth-thermogram curves of Escherichia coli (E. Coli) affected by Shexiang (Moschus) and Bingpian (Beolum Synthcticum) at different concentrations were determined with microcalorimetry, and the power-time curves (thermogram curves) of E. Coli metabolism and characteristic parameters, such as growth rate constant (k), maximum output power (Pm), peak time (tp), total heat-output (Qt), etc. Were analyzed with the principal component analysis (PCA) to find the close correlative parameters, so as to objectively reflect the drug property differences of Shexiang (Moschus) and Bingpian (Beolum Synthcticum).Results: The values of P2 in the second exponential growth phase increased with the increase of the concentrations of Shexiang (Moschus) and Bingpian (Beolum Synthcticum), and the P2 of Shexiang (Moschus) was larger than that of Bingpian (Beolum Synthcticum); Q2 increased with the increase of the concentrations of Shexiang (Moschus), but for Bingpian (Beolum Synthcticum) it was opposite. It is indicated that they have different effects on P2 and Q2 of E. Coli in the second exponential growth phase, and have differences in warm and cold natures.Conclusion: The microcalorimetry can accurately and objectively appraise differences of the drug property of Shexiang (Moschus) and Bingpian (Beolum Synthcticum) and verify the objectivity and authenticity of the drug property, so as to provide a new and useful method for studies of the drug property of Chinese drugs.