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目的探讨红细胞悬液延迟输注30 min对大鼠失血性休克复苏的影响。方法 24只Wistar大鼠随机分为假手术(SHAM)、RBC 1和RBC 2组(n=8),RBC 1和RBC 2组制备重度失血性休克大鼠模型,先后采用晶胶复合液和红细胞悬液复苏,RBC 2组红细胞悬液延迟输注30 min;监测生理、血气等指标。结果与RBC1组相比,RBC 2组平均动脉压(MAP)和体温恢复延迟,RBC 1组复苏结束后2组MAP分别为(116.99±11.06)和(73.72±14.34)mm Hg(P<0.01);RBC 1组复苏结束后30 min,2组MAP分别为(103.07±9.59)和(120.61±10.73)mmHg(P<0.01)。RBC 1组复苏结束后120 min RBC 1组体温(37.28±0.80)℃明显低于RBC 2组(38.83±0.58)℃(P<0.01)。与SHAM组相比,RBC 1和RBC 2组休克后pH、PCO2、BE和ctHb明显降低,PO2明显升高;复苏后PCO23 h,RBC 1和RBC 2组碱剩余(BE)值明显低于SHAM组。结论在失血性休克液体复苏的基础上,红细胞悬液延迟输注30 min对失血性休克大鼠模型的生理、血气等指标无明显影响。提示紧急救治中输血准备工作耗时可能不影响失血性休克的复苏效果。
Objective To investigate the effect of delayed infusion of erythrocyte suspension on hemorrhagic shock and resuscitation in rats. Methods Twenty-four Wistar rats were randomly divided into sham operation (SHAM), RBC 1 and RBC 2 groups (n = 8), RBC 1 and RBC 2 groups were given severe hemorrhagic shock rats model, Suspension resuscitation, RBC 2 groups of red blood cell suspension delayed infusion of 30 min; monitoring of physical, blood gas and other indicators. Results Compared with RBC1 group, mean arterial pressure (MAP) and body temperature recovery were delayed in RBC 2 group. The MAP of the two groups after RBC 1 resuscitation were (116.99 ± 11.06) and (73.72 ± 14.34) mm Hg, respectively ; MAP of the two groups were (103.07 ± 9.59) and (120.61 ± 10.73) mmHg respectively (P <0.01) 30 minutes after the resuscitation of RBC 1 group. The body temperature of RBC 1 group (37.28 ± 0.80) ℃ was significantly lower than that of RBC 1 group (38.83 ± 0.58) ℃ (P <0.01) 120 minutes after the end of RBC 1 resuscitation. Compared with SHAM group, the values of pH, PCO2, BE and ctHb were significantly decreased and PO2 was significantly increased in RBC1 and RBC2 groups after shock. The values of BE in PCO23 h, RBC1 and RBC2 groups after resuscitation were significantly lower than those in SHAM group group. Conclusion On the basis of hemorrhagic shock fluid resuscitation, delayed infusion of erythrocyte suspension for 30 min has no significant effect on the indexes of physiological and blood gas in hemorrhagic shock rats. Prompt emergency blood transfusion preparation time-consuming may not affect the recovery of hemorrhagic shock.