论文部分内容阅读
目的研究肺癌患者外周血中CEAmRNA、CK19mRNA和CK20mRNA的表达及联合检测的临床意义。方法采用荧光定量PCR技术,检测48例肺癌患者、8例肺良性病变和30名健康志愿者外周血CEAmRNA、CK19mRNA和CK20mRNA水平。结果48例肺癌患者外周血CEAmRNA的水平为(16264±28765)copies/ml,其中26例阳性,阳性率为54.2%;CK19mRNA的水平为(14891±27244)copies/ml,其中27例阳性,阳性率为56.3%;CK20mRNA的水平为(10924±21678)copies/ml,其中20例阳性,阳性率为41.7%。8例肺良性病变中CK20mRNA阳性1例(12.5%),CEAmRNA和CK19mRNA均为阴性,健康志愿者30例未见表达。单项检测敏感度和准确性较低,联合其中二项检测敏感度和准确性较高,联合三项敏感度达77.1%、准确性可达87.2%。CEAmRNA、CK19mRNA和CK20mRNA水平和阳性率与肺癌类型和不同临床分期有关。结论CEAmRNA、CK19mRNA和CK20mRNA可作为标志物检测肺癌患者外周血微转移,联合二至三项指标有较高的检出率,定量检测有助于肺癌微转移的监测。
Objective To study the clinical significance of combined detection of CEAmRNA, CK19mRNA and CK20mRNA in peripheral blood of patients with lung cancer. Methods Real-time fluorescence quantitative PCR was used to detect the levels of CEAmRNA, CK19mRNA and CK20mRNA in peripheral blood of 48 lung cancer patients, 8 benign pulmonary lesions and 30 healthy volunteers. Results The level of CEAmRNA in peripheral blood of 48 patients with lung cancer was (16264±28765) copies/ml, of which 26 were positive, the positive rate was 54.2%; the level of CK19mRNA was (14891±27244) copies/ml, of which 27 were positive and positive. The rate was 56.3%; the level of CK20 mRNA was (10924±21678) copies/ml, of which 20 cases were positive and the positive rate was 41.7%. In 8 benign lung lesions, 1 case (12.5%) was positive for CK20 mRNA, CEA mRNA and CK19 mRNA were negative, and no expression was found in 30 healthy volunteers. Sensitivity and accuracy of the individual items were low, and sensitivity and accuracy of the two items in the combination were high. The combined sensitivity of the three items was 77.1% and the accuracy was 87.2%. The levels and positive rates of CEA mRNA, CK19 mRNA and CK20 mRNA were related to the type of lung cancer and different clinical stages. Conclusion CEAmRNA, CK19mRNA and CK20mRNA can be used as markers to detect peripheral blood micrometastasis in patients with lung cancer. Combined detection of two to three indicators has a higher detection rate. Quantitative detection is helpful for the monitoring of micrometastasis in lung cancer.