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目的 了解人类免疫缺陷病毒 1型E亚型毒株在深圳市不同人群中流行传播情况、流行时间和传播规律。方法 应用聚合酶链反应 (PCR)方法对 1996年深圳市检出的 3份人类免疫缺陷病毒 1型 (HIV 1)感染者外周血单个核细胞样本进行扩增 ,获得HIV 1膜蛋白 (env)基因片段 ,并对C2 V3及其邻区 35 0～ 45 0个核苷酸序列进行测定和分析。结果 这 3份血样为HIV 1E亚型毒株感染 (sz E) ,彼此间的基因离散率为 2 .6 % ;与A E国际参考亚型及国内部分地区流行的BE型代表株比较 ,sz E与A D参考亚型共享序列及国内B亚型代表株间的基因离散率均大于 2 4% ,而与主要代表泰国E亚型 (Econ)间的基因离散率仅为 6 .2 %。系统树分析显示 ,sz E与Econ聚集在一起 ,远离其他国际亚型毒株序列。结论 HIV 1E亚型在深圳的流行时间约为 2～ 3年 ,主要经共用针具等行为首先在静脉药瘾人群中流行 ,且具有向职业献血员中传播蔓延的潜在危险。
Objective To understand the prevalence, prevalence and transmission of human immunodeficiency virus type 1 E subtype strains in different populations in Shenzhen. Methods Peripheral blood mononuclear cells (PBMCs) from 3 HIV-1 infected patients were detected by polymerase chain reaction (PCR) in 1996 in Shenzhen. HIV 1 membrane protein (env) Gene fragments, and the C2 V3 and its neighboring 35 0 ~ 45 0 nucleotide sequence determination and analysis. Results The three blood samples were infected with HIV subtype 1E (sz E) and their gene dispersal rate was 2.6%. Compared with the international reference strains of AE and the representative strains of BE type prevailing in some parts of China, sz E The genetic divergence between the shared sequences with the reference reference subtype AD and the domestic representative subtype B subtypes was greater than 24%, while the genetic divergence with the major representative Thai E subtype was only 6.2%. Phylogenetic tree analysis showed that sz E and Econ clustered together away from other international subtype strains. Conclusion The prevalence of HIV 1E subtype in Shenzhen is about 2 ~ 3 years. The prevalence of HIV 1E subtype is mainly prevalence in the intravenous addicts mainly through the sharing of needles and other diseases and has the potential of spreading to professional blood donors.