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目的探讨高糖诱导下曲古霉素A(TSA)和5-氮杂胞苷(5-AzaC)对胰岛β细胞增殖、凋亡及功能的影响。方法体外培养大鼠胰岛素瘤(RIN-m5f)细胞至指数增长期,根据干预方案分为空白对照(NC)组、高糖诱导(HG)组、0.05、0.10μmol/L TSA干预组、0.63、1.25μmol/L 5-AzaC干预组、0.10μmol/L TSA+1.25μmol/L 5-AzaC联合干预组。检测RIN-m5f细胞增殖活性、细胞凋亡率、葡萄糖刺激胰岛素分泌能力。结果 0.05、0.10μmol/L TSA干预组、0.63、1.25μmol/L 5-AzaC干预组及联合干预组的细胞增殖活性均高于HG组,而细胞凋亡率均低于HG组(P<0.05)。在3.3mmol/L及25mmol/L葡萄糖刺激时,各组葡萄糖刺激胰岛素分泌量均高于HG组(P<0.05)。结论TSA和5-AzaC可抑制高糖诱导的胰岛β细胞凋亡和恢复β细胞的胰岛素分泌功能。
Aim To investigate the effects of TSA and 5-AzaC on the proliferation, apoptosis and function of islet β cells induced by high glucose. Methods The rat insulinoma (RIN-m5f) cells were cultured in exponential phase. According to the intervention protocol, they were divided into control group (NC), HG group, 0.05,0.10μmol / L TSA intervention group 1.25μmol / L 5-AzaC intervention group, 0.10μmol / L TSA + 1.25μmol / L 5-AzaC intervention group. Detection of RIN-m5f cell proliferation activity, apoptosis rate, glucose stimulated insulin secretion. Results Compared with HG group, the cell proliferation activity in the groups treated with 0.05,0.10μmol / L TSA, 0.63,1.25μmol / L 5-AzaC and the combination intervention group were higher than those in the HG group (P <0.05) ). Glucose-stimulated insulin secretion in each group was significantly higher than that in HG group (P <0.05) at 3.3mmol / L and 25mmol / L glucose stimulation. Conclusion TSA and 5-AzaC can inhibit high glucose-induced pancreatic β-cell apoptosis and restore β-cell insulin secretion.