论文部分内容阅读
目的探讨原位杂交技术在真菌性鼻窦炎病原学诊断方面的应用价值。方法应用针对曲霉菌属18S rRNA 序列互补的18S-1寡核苷酸探针,经地高辛标记对37例真菌性鼻窦炎患者术后的石蜡包埋标本进行原位杂交检测。结果 37例真菌性鼻窦炎标本中28例曲霉菌属原位杂交呈阳性反应,作为对照的白色念珠菌感染组织杂交结果为阴性。HE 染色和六胺银染色可疑曲霉菌感染分别为21例和23例。结论真菌性鼻窦炎真菌培养阴性或无法进行真菌培养时,原位杂交是一种敏感、特异和快速检测组织内真菌病原菌的有效方法。
Objective To investigate the value of in situ hybridization in the etiological diagnosis of fungal sinusitis. Methods 18S-1 oligonucleotide probes complementary to the 18S rRNA sequence of Aspergillus were used to detect the paraffin-embedded specimens of 37 patients with fungal sinusitis by digoxin labeling. Results The results of in situ hybridization of 28 Aspergillus strains in 37 specimens of fungal sinusitis were positive. The result of hybridization of Candida albicans infected tissues was negative. Susceptible Aspergillus infections by HE staining and silver hexamine were 21 and 23, respectively. Conclusion In situ hybridization is a sensitive, specific and rapid method for the detection of fungal pathogens in tissues when fungal sinusitis is fungal negative or inability to culture.