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利用 Co6 0 - γ辐照与 Na N3(Sodium azide)复合处理及成熟胚培养的方法 ,诱发不育性稳定的籼型光敏核不育系HN5 s产生可育突变体 ,通过不同突变体的自交 ,相互杂交及其与 HN5 s测交 ,并在人工控制光温条件下鉴定不育性 ,研究 HN5 s不育性的遗传。结果显示 :在人工诱变的条件下 ,HN5 s可以产生育性恢复和长日高温不育而长日低温可育两类可育突变体 ,HN5 s的不育性受两对隐性核基因控制 ,其中一对不育基因的作用较强 ,另一不育基因的作用较弱。当作用强的不育基因突变为作用弱的不育基因时 ,光敏不育系转变为温敏不育系。推断光敏核不育性的稳定性与不育系 (或品种 )对自然诱变因素的敏感性有关。同时本文还讨论了光敏核不育系不育性不稳定的原因及选育稳定的光敏核不育系时应注意的问题
The fertile mutant HN5-S was induced by Co6 0 - γ irradiation combined with Na N3 (Sodium azide) treatment and mature embryo culture, and the fertile mutant HN5 s was induced by different mutants Hybridization and cross detection with HN5 s, and sterility was identified under artificial light and temperature conditions to study the inheritance of HN5 s infertility. The results showed that under artificial mutagenesis conditions, HN5s could produce fertile restorers and long-day and high-temperature sterile fertile two long-day and low-fertile fertile mutants. The sterility of HN5s was affected by two pairs of recessive nuclear genes Control, one pair of sterility gene role is strong, the effect of another sterile gene is weaker. When a strong sterile gene is mutated to a weakly sterile gene, the photo-sensitive male sterile line is transformed into a thermo-sensitive male sterile line. It is deduced that the stability of photosensitive nuclear sterility is related to the sensitivity of CMS (or breed) to natural mutagenic factors. At the same time, this paper also discussed the reasons of instability of the photosynthetic male sterile lines and the problems that should be noticed when breeding the stable photosensitive genic male sterile lines