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目的 观察慢性次声暴露下 ,其豚鼠耳蜗毛细胞听毛形态学的影响 .方法 豚鼠 (n=2 0 )在接受次声暴露条件为 8Hz,12 0 d B SPL ,1次· d- 1 ,每次 2 h、共 30 d的暴露后 ,快速活杀并制备耳蜗硬铺片银浸标本 ,通过光学显微镜观察豚鼠耳蜗毛细胞听毛的变化情况 .结果 出现了以外毛细胞为主的耳蜗毛细胞听毛损伤 ,且由底周至顶周外毛细胞听毛的损伤逐渐加重 ,表现为听毛水肿及部分倒伏、缺失 ,失去“V”字外形 ,呈碎屑样杂乱无章 ,排列混乱 ;空旷的表皮板排列亦明显不齐 .结论 慢性次声暴露对外周听觉系统具有明确的损伤作用 ;推测耳蜗毛细胞损伤的机械性因素占主导地位 ,声学性损伤因素为辅 ,这与可听声的听觉系统损伤机制比较是有差异的
Objective To observe the morphological changes of cochlear hair cells in guinea pigs under chronic infrasound exposure.Methods Guinea pigs (n = 20) were exposed to infrasound for 8 Hz, 120 d B SPL, 1 d · 1, After exposure for 2 days and exposure to 30 d each time, the cochlear hard-coated silver immersion specimens were quickly killed and prepared, and the changes of the auditory hair of guinea pig cochlear hair cells were observed by light microscope. Cells to listen to hair damage, and from the end of the week to the top of the outer hair cells to listen to the hair damage gradually increased, manifested as listening to Mao Edema and some lodging, loss, loss of “V” shape was debris-like clutter chaos arranged; open The arrangement of epidermis was also obviously irregular.Conclusion Chronic infrasound exposure has obvious damage to the peripheral auditory system.It is presumed that the mechanical factors of cochlear hair cell damage are dominant and the acoustic damage factors are supplemented.This is consistent with auditory audible System damage mechanism is different