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LAK 细胞是免疫治疗中的一种效应细胞,已用于肿瘤治疗。了解冷冻保存对LAK 细胞活性的影响,在免疫治疗、诊断和疗效评价也很重要。但已往实验对冷冻后NK、K 和T 细胞保持细胞毒活性的能力仍有争议。本研究的目的是进一步实验比较冷冻前后经rIL-2激活外周单个核细胞(PBMC)形成的LAK 细胞和不经rIL-2激活的NK 细胞的活性,同时观察在实验过程中细胞表面标记表达的变化。实验方法:利用Cs3000型细胞分离器和Ficoll
LAK cells are a type of effector cell in immunotherapy and have been used in cancer therapy. Understanding the effect of cryopreservation on the activity of LAK cells is also important in immunotherapy, diagnosis, and efficacy evaluation. However, the ability of previous experiments to maintain cytotoxic activity of NK, K and T cells after freezing remains controversial. The purpose of this study was to further compare the activities of LAK cells activated by rIL-2 activated peripheral blood mononuclear cells (PBMCs) and NK cells not activated by rIL-2 before and after freezing, and observe the expression of cell surface markers during the experiment Variety. Experimental Methods: Using Cs3000 Cell Separator and Ficoll