论文部分内容阅读
目的：探讨海蛇乙醇浸出物（ＡＥＢＦＳＳ）灌胃对大鼠胶原性关节炎（ＣＩＡ）的预防和治疗作用。方法：用自制的可溶性猪尾Ⅱ型胶原注入实验大鼠左后足造成胶原诱导性大鼠关节炎模型，观察用（预防性或治疗性）与不用ＡＥＢＦＳＳ对ＣＩＡ的影响。结果：在致炎前ＡＥＢＦＳＳ灌胃，当剂量达浸出蛋白４．０ｍｇ／ｋｇ，可明显推迟ＣＩＡ发病时间，降低ＣＩＡ患病率和减轻关节炎症状；在致炎后ＡＥＢＦＳＳ灌胃，可明显减轻关节炎症的程度，其作用在用药７天后开始表现明显。关节组织病理检查显示，ＡＥＢＦＳＳ显著减轻了患鼠关节的炎症程度。初步分析表明，ＡＥＢＦＳＳ灌胃既降低了ＣＩＡ患鼠血清抗ＣⅡ抗体水平，又对患鼠耳迟发型超敏反应（ＤＴＨ）产生了明显的抑制作用。结论：ＡＥＢＦＳＳ灌胃对大鼠胶原性关节炎有显著的防治作用；而其机理同时涉及Ｔ和Ｂ淋巴细胞免疫。
Objective: To investigate the preventive and therapeutic effects of intragastric administration of AEBFSS on collagen induced arthritis (CIA) in rats. Methods: A collagen-induced arthritis model was induced in the left hind leg of experimental rats by injecting soluble porcine collagen Ⅱ. The effects of (prophylactic or therapeutic) and non-AEBFSS on CIA were observed. Results: Before AEBFSS was given into the stomach, the leaching protein 4.0mg / kg could significantly delay the onset of CIA, reduce the prevalence of CIA and reduce the symptoms of arthritis. After AEBFSS was given into the stomach, it could be obviously relieved The degree of joint inflammation, its role in the medication started 7 days after the obvious. Arthroscopic histopathology showed that AEBFSS significantly reduced the degree of inflammation in the affected joints. Preliminary analysis showed that the administration of AEBFSS not only reduced the level of anti-CⅡantibody in CIA mice, but also inhibited the delayed-type hypersensitivity (DTH) in mice. Conclusion: The intragastric administration of AEBFSS has a significant preventive and therapeutic effect on collagen-induced arthritis in rats, and its mechanism involves both T and B lymphocyte immunization.