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目的研究胃窦肌间神经丛中单胺氧化酶mRNA表达,探讨5羟色胺(5-HT)在胃起搏机制中的作用。方法通过手术建立Wistar大鼠胃起搏模型(近远端胃各缝制一对电极),分为起搏组(n=10)和对照组(n=6)。采用逆转录聚合酶链反应(RT-PCR)检测起搏组和对照组胃窦肌间神经丛中MAO-A mRNA、MAO-B mRNA的表达量,以恒定表达的β-actin作为内参照。计算MAO-AmRNA、MAO-B mRNA与β-actin mRNA表达积分光密度值的比例(MA/B,MB/B),以反映组织中MAO-AmRNA、MAO-B mRNA的相对表达量。结果RT-PCR研究显示起搏组MAO-A mRNA表达显著弱于对照组,起搏组MA/B比值明显较对照组较少(0.37±0.11vs0.95±0.57,P<0.001);而起搏组MAO-B mRNA表达与对照组无差别,两组间MB/B比值比较也无显著性意义(0.97±0.24vs1.01±0.58,P>0.05)。结论胃起博后胃肌间神经丛内单胺氧化酶mRNA表达量明显减少,表明胃窦肌间神经丛内5-HT可能在胃起搏中发生了重要作用。
Objective To study the expression of monoamine oxidase mRNA in the myenteric plexus of the antrum and to explore the role of 5-hydroxytryptamine (5-HT) in gastric pacing mechanism. Methods The gastric pacing model of Wistar rats was established by surgery. A pair of electrodes were made in the proximal and distal stomach, which were divided into pacing group (n = 10) and control group (n = 6). The expression of MAO-A mRNA and MAO-B mRNA in the pyloric myenteric plexus of pacing group and control group were detected by reverse transcription-polymerase chain reaction (RT-PCR), and the constant expression of β-actin was used as internal reference. The ratio of MAO-AmRNA, MAO-B mRNA and β-actin mRNA expression integral optical density (MA / B, MB / B) was calculated to reflect the relative expression of MAO-AmRNA and MAO-B mRNA in the tissue. Results RT-PCR showed that MAO-A mRNA expression in pacing group was significantly weaker than that in control group (P <0.001) The MAO-B mRNA expression in stroke group was not different from that in control group, and there was no significant difference in MB / B ratio between the two groups (0.97 ± 0.24vs1.01 ± 0.58, P> 0.05). Conclusion The mRNA expression of monoamine oxidase in gastric myenteric plexus decreased significantly after gastric piraging, indicating that 5-HT in gastric myenteric plexus may play an important role in gastric pacing.