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本文从大豆膜质过氧化反应及其保护酶体系的变化、过氧化物酶和苹果酸脱氢酶同工酶的表达特性,分析了不同类型的大豆品种对大豆病毒病1号生理小种(SMV1)侵染后的生理生化反应。研究结果表明:大豆品种经SMV1号株系诱导后,感病品种丙二醛(MDA)、超氧化物歧化酶(SOD)活性增加幅效较大,抗病品种增加较少。过氧化氢酶(CAT)活性的表达则相反,在花叶病毒侵染条件下,感病与抗病品种过氧化物酶和苹果酸脱氢酶同工酶的表达存在着明显的差异。感病品种,过氧化物同工酶活性增强,并产生了一些大分子酶蛋白,苹果酸脱氢酶同工酶活性也明显增强;而抗病品种的过氧化物酶及苹果酸脱氢酶同工酶的表达则没有改变,只是过氧化物酶同工酶的活性有所增强。植株体内的生理生化反应和变化与品种的抗性相一致。
In this paper, the changes of membrane lipid peroxidation in soybean and its protective enzyme system, the expression characteristics of peroxidase and malate dehydrogenase isoenzymes were analyzed, and the effects of different types of soybean on soybean virus disease 1 race Physiological and biochemical reactions after SMV1 infection. The results showed that the soybean varieties induced by SMV1 strain, the susceptible varieties of malondialdehyde (MDA), superoxide dismutase (SOD) increased efficiency, resistance varieties increased less. On the contrary, the expression of catalase (CAT) activity showed a significant difference in the expression of peroxidase and malate dehydrogenase isozymes between susceptible and resistant cultivars under mosaic virus infection. Susceptible varieties, peroxidase isozyme activity increased, and some macromolecular enzyme protein, malate dehydrogenase isozyme activity was significantly enhanced; and resistant varieties of peroxidase and malate dehydrogenase Isoenzyme expression did not change, but peroxidase isozyme activity has increased. Physiological and biochemical reactions and changes in plants are consistent with the variety resistance.