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目的检测不同神经系统发育阶段铅暴露对仔鼠海马组织中多聚唾液酸转移酶STX基因m RNA转录水平的影响,探讨铅暴露对仔鼠神经系统发育损伤的可能机制。方法实时荧光定量PCR(real-time q PCR)检测各实验组出生后1 d、3周及6周仔鼠海马组织中STX基因m RNA转录水平。结果正常Wistar大鼠海马组织中STX基因m RNA的转录水平随着仔鼠生长发育逐渐降低;母铅组1日龄仔鼠与对照组相比,STX基因m RNA转录水平明显降低,差异有统计学意义(P<0.05);母铅组3周龄仔鼠、断乳后染铅组6周龄仔鼠分别与其对照组相比,STX基因m RNA转录水平差异无统计学意义(P>0.05);母铅6周龄仔鼠与断乳后6周龄各染铅组、对照组相比,STX基因m RNA转录水平明显升高,差异有统计学意义(P<0.05),各时间点仔鼠1 mmol/L与2 mmol/L染铅组相比,STX基因m RNA转录水平差异无统计学意义(P>0.05)。结论不同神经系统发育阶段,低剂量铅暴露影响仔鼠海马组织中STX基因m RNA转录水平,可能是铅暴露损害神经系统发育的原因。
Objective To detect the effects of lead exposure at different developmental stages of the nervous system on the m RNA transcription level of polysialic acid transferase STX gene in the hippocampus of neonatal rats and to explore the possible mechanism of lead exposure on the neuronal development of the offspring. Methods Real-time q-PCR was used to detect the transcription level of STX mRNA in hippocampus of the experimental groups at 1, 3, and 6 weeks after birth. Results The transcription level of STX m RNA in the hippocampus of normal Wistar rats decreased gradually with the growth of offspring. Compared with the control group, the transcriptional level of m RNA of STX gene in the first-day offspring of lead group was significantly lower (P <0.05). Compared with the control group, there was no significant difference in mRNA expression levels of STX gene between the three-week-old offspring of lead group and offspring of lead poisoning group at 6 weeks (P> 0.05 ). Compared with the control group, the m RNA transcription level of STX gene was significantly increased in 6-week old offspring offspring and 6 weeks postweaning offspring. The difference was statistically significant (P <0.05). At each time point Compared with 2 mmol / L lead exposure group, there was no significant difference in m RNA transcription level of STX gene between offspring 1 mmol / L (P> 0.05). Conclusions In different stages of the nervous system, the low dose lead exposure may affect the transcription level of STX mRNA in the hippocampus of the offspring. This may be the reason that the lead exposure damages the nervous system.