论文部分内容阅读
目的对一起食物中毒样品进行致病菌检测,准确分析食物中毒原因,为食物中毒的处理提供依据。方法依据《食品卫生微生物学检验》国家标准GB4789.10-2010、GB 14938-94《食物中毒诊断标准及技术处理总则》和WS/T80-1996《葡萄球菌食物中毒诊断标准及处理原则》方法对采集的12份食物中毒样品进行病原菌金黄色葡萄球菌检测;应用荧光定量PCR法对检出的金黄色葡萄球菌进行肠毒素检测。结果在12份样品中检出5株金黄色葡萄球菌,分别来自患者呕吐物4份,砧板棉拭子1份,全部检出A型肠毒素,未检出其他病原菌。金黄色葡萄球菌总检出率为41.7%。结论金黄色葡萄球菌A型肠毒素是此次食物中毒的病原体。实时荧光PCR快速检测方法适用于金黄色葡萄球菌肠毒素的基因分型。
Objective To detect pathogenic bacteria in food poisoning samples and analyze the causes of food poisoning accurately so as to provide the basis for the treatment of food poisoning. Methods According to the national standards GB4789.10-2010 and GB 14938-94 of “Microbiological examination of food hygiene” and the “General principles of diagnostic criteria and technical treatment of food poisoning” and WS / T80-1996 “Diagnostic criteria and treatment principles of staphylococcal food poisoning” Staphylococcus aureus was detected in 12 samples of food poisoning. Staphylococcus aureus was detected by real-time PCR. Results Five strains of Staphylococcus aureus were detected in 12 samples, including 4 samples of vomit and 1 cotton swab of chopping block, respectively. All of the samples were positive for type A enterotoxin but no other pathogens were detected. The total detection rate of Staphylococcus aureus was 41.7%. Conclusion Staphylococcus aureus enterotoxin is the pathogen of food poisoning. Real-time fluorescent PCR rapid detection method for Staphylococcus aureus enterotoxin genotyping.