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利用固体超强酸SO2-4/TiO2催化合成反丁烯二酸蔗糖甲酯(SMF),反应分为3步:马来酸酐先和蔗糖反应生成顺丁烯二酸单蔗糖酯、顺丁烯二酸单蔗糖酯异构化和反丁烯二酸单蔗糖酯与甲醇酯化反应生成SMF。用单因素法确定了“一锅法”合成SMF的工艺。研究了酯化度为5.5的SMF的最低抑菌浓度和生长抑制曲线,并与山梨酸及富马酸二甲酯进行了对比。结果表明:SMF具有较广的抗菌谱系,对细菌、酵母和霉菌的生长具有较强的抑制能力。添加0.12%的SMF可使大肠杆菌和苏云金杆菌的生长适应期分别由对照的3h延长至30h和35h;添加0.10%的SMF能使酵母的生长适应期延长至对照(5h)的6倍,添加0.06%SMF,液体培养96h后才有黑曲霉菌丝球出现,而对照样20h即可见到。反丁烯二酸蔗糖甲酯的抑菌效果好于山梨酸而与富马酸二甲酯接近。
The solid superacid SO2-4 / TiO2 was used to synthesize sucrose fumarate methyl ester (SMF). The reaction was divided into three steps: maleic anhydride first reacted with sucrose to form maleic acid mono-sucrose ester, maleic acid bis Isomerisation of acid mono-sucrose ester and esterification of fumaric acid fumarate with methanol give SMF. Using single factor method to determine the “one pot method” SMF synthesis process. The minimum inhibitory concentrations and growth inhibition curves of SMF with the degree of esterification of 5.5 were studied and compared with sorbic acid and dimethyl fumarate. The results showed that: SMF has a wide range of antibacterial lineages and has strong inhibitory effects on the growth of bacteria, yeasts and molds. Adding 0.12% SMF could prolong the growth adaptability of Escherichia coli and Bacillus thuringiensis from the control 3h to 30h and 35h, respectively. Adding 0.10% SMF could prolong the growth adaptability of yeast to 6 times of the control (5h) 0.06% SMF, Aspergillus niger skeletal appeared after 96h liquid culture, but the control could be seen in 20h. Bacteriostasis of fumaric acid fumarate was better than that of sorbic acid and dimethyl fumarate.