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通过对CP1抗菌蛋白作用下出芽短梗霉胞内蛋白质及离子的泄漏进行研究表明,CP1抗菌蛋白引起菌丝和孢子蛋白质的泄漏较明显,CP1抗菌蛋白可直接引起菌丝体和孢子的外壁穿孔、破裂,胞内物质泄漏;CP1抗菌蛋白还能引起菌丝和孢子内Ca2+的泄漏,说明该抗菌蛋白能作用于出芽短梗霉菌丝体和孢子外膜,形成离子通道,使Ca2+从菌体细胞中释放出来.CP1抗菌蛋白对出芽短梗霉菌丝体和孢子的氧呼吸有明显的抑制作用,随着CP1抗菌蛋白浓度的增加,其耗氧百分率减少,呼吸作用从起始到停止经历的时间缩短.表明CP1抗菌蛋白对出芽短梗霉菌丝体和孢子氧呼吸的抑制作用是其活性作用的一个重要原因.
The results showed that the CP1 antimicrobial protein caused obvious leakage of mycelium and spore protein, CP1 antimicrobial protein can directly lead to the perforation of the outer wall of mycelium and spores , Rupture, intracellular material leakage; CP1 antimicrobial protein can also cause Ca2 + leakage in mycelium and spores, indicating that the antimicrobial protein can act on mycelium and spore adventitia pullulans to form ion channels, Ca2 from the bacteria Cells.CP1 antimicrobial protein significantly inhibited the oxygen respiration of A. pullulans mycelia and spores, with the increase of CP1 antibacterial protein concentration, the oxygen consumption decreased, the respiration from the start to stop experienced The time was shortened.The results showed that the inhibitory effect of CP1 antimicrobial protein on the mycelium and spore oxygen respiration of A. pullula was an important reason for its activity.