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目的 研究乳腺癌血管生成与转移抑制基因nm2 3 H1的表达在肿瘤转移中的作用 ,分析它们与腋窝淋巴结转移的关系。 方法 对 80例乳腺癌患者采用能量多普勒检测肿瘤内血流信号并通过图像分析技术定量测定肿瘤内血管阳性反应总面积 ,对手术切除标本采用免疫组织化学技术检测nm2 3基因蛋白及肿瘤内微血管密度 (microvesseldensity,MVD)的表达。 结果 伴有腋窝淋巴结转移组 (lymphnodepostive ,LN +)肿瘤血管阳性反应总面积、MVD值明显高于不伴有腋窝淋巴结转移组 (lymphnodenegative ,LN - ) (t=7 0 7、6 34,P <0 0 1) ,而nm2 3 H1的表达则相反 ,同时nm2 3 H1阴性表达组的血管阳性反应总面积、MVD值显著高于nm2 3 H1阳性表达组 ,(t =6 86、6 4 9,P <0 0 5 )。 结论 肿瘤血管生成和nm2 3 H1的表达在乳腺癌腋窝淋巴结转移过程中发挥着重要作用。nm2 3 H1基因表达与乳腺癌血管生成可能存在某种内在联系。
Objective To study the role of nm23 H1 in the expression of angiogenesis and metastasis suppressor gene in breast cancer and to investigate the relationship between them and axillary lymph node metastasis. Methods Eighty patients with breast cancer were examined for the intracranial flow signals by energy Doppler and quantitatively determined the total area of intravascular tumor-positive reaction by image analysis technique. Immunohistochemistry was used to detect the expression of nm23 protein and tumor in the resected specimens Microvessel density (MVD) expression. Results The total area of blood vessel positive reaction in patients with lymphnodepostive neoplasm (LN +) was significantly higher than those without lymph node metastasis (LN - t = 707,634, P < While the expression of nm23 H1 was opposite, while the total area of vascular positive reaction and the MVD in nm23 H1 negative group were significantly higher than those in nm23 H1 positive group (t = 6 86, 649, P <0 0 5). Conclusion Tumor angiogenesis and nm23 H1 expression play an important role in axillary lymph node metastasis of breast cancer. nm23 H1 gene expression and breast cancer angiogenesis may be some intrinsic link.