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目的 建立鼠慢性萎缩性胃炎 (CAG)动物模型并探讨致萎缩因素。方法 应用① 6 0 %酒精和 2 0mmol/L去氧胆酸钠 (A因素 ) ;② 0 .0 5 %～ 0 .1%氨水 (B因素 ) ;③ 0 .0 5 %吲哚美辛 (C因素 )按有重复的L8(2 7)正交设计对SD大鼠分三批进行制模刺激 ,三批刺激时间分别为 3个月、6个月和 9个月。实验到期后取出全胃 ,观察大鼠胃黏膜大体观及各项萎缩指标。结果 应用A、B、C三因素 (单因素或多因素 )刺激 6个月或 9个月均出现典型的胃窦萎缩性胃炎征象 ,制模成功率达 10 0 % ,且能维持形态稳定 1个月以上。结论 应用 6 0 %酒精和 2 0mmol/L去氧胆酸钠 ,0 .0 5 %～ 0 .1%氨水 ,0 .0 5 %吲哚美辛对SD大鼠刺激 6个月可成功地建立CAG模型 ,且稳定性良好
Objective To establish an animal model of chronic atrophic gastritis (CAG) in rats and to explore the factors that cause atrophy. Methods ① 60% alcohol and 20 mmol / L sodium deoxycholate (A factor); ② 0.05% -0.1% ammonia (B factor); ③ 0. 0 5% indomethacin C factors) according to repeated L8 (2 7) orthogonal design of SD rats in three batches of modeling stimuli, three batches of stimulation time were 3 months, 6 months and 9 months. After the expiration of the experiment, the whole stomach was taken out to observe the general concept of gastric mucosa and the indicators of atrophy. Results Antral atrophic gastritis signs appeared typical at 6 months or 9 months after application of A, B and C factors (single factor or multifactorial factors), the success rate of molding was 100% and the morphological stability was maintained More than a month. CONCLUSIONS: Six months of 6-month stimulation of SD rats with 60% alcohol and 20 mmol / L sodium deoxycholate, 0.05% -0.1% ammonia, and 0.05% indomethacin can be successfully established CAG model, and the stability is good