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目的 :研究鸡蛋清溶菌酶 (L ZM)与头孢他啶 (CFT)联合应用对痢疾杆菌的生长、形态及其致炎活性物质释放的影响。方法 :在营养肉汤或稀释血液培养的痢疾杆菌液中加入 L ZM或 CFT作用一定时间后 ,采用营养琼脂平板接种 ,计数各培养管中存活细菌数。将细菌悬液离心 ,收集培养上清液 ,将上清液加入到培养的巨噬细胞中 ,测定其对巨噬细胞炎性因子如肿瘤坏死因子 α(TNFα)、一氧化氮 (NO)的诱生能力 ;同时用光学显微镜观察各培养管中细菌的形态。结果 :营养肉汤培养中的痢疾杆菌在 CFT(16× MIC、32× MIC)作用下 ,可造成细菌迅速溶解 ,同时伴有大量致炎活性物质释放 ,其培养上清在体外培养的巨噬细胞上可诱导大量的TNFα、NO产生 ;L ZM与 CFT联合使用既能阻止细菌溶解 ,又能保持与 CFT单独用药相当的杀菌作用 ;镜下观察 ,死亡的细菌相互粘连 ,形成聚集体 ;联合用药后可降低巨噬细胞的激活 ,减少 TNFα、NO的产生。在血液培养系统中 ,CFT(16× MIC、32× MIC)对痢疾杆菌有较强的杀菌作用 ,但较在肉汤培养体系中弱 ;L ZM与 CFT联合用药较 CFT(16× MIC、32× MIC)单独用药有更强的杀菌作用 ,并能显著降低 NO的释放。结论 :L ZM与 CFT联合应用能阻止痢疾杆菌溶解 ,降低细菌致炎活性物质 (主要为内毒素 )的释放
Objective: To study the effects of egg white lysozyme (L ZM) combined with ceftazidime (CFT) on the growth, morphology and release of inflammatory active substances of Shigella dysenteriae. Methods: LZM or CFT was added to the broth of dysentery bacilli in nutrient broth or diluted blood for a certain period of time, and then the nutrient agar plates were inoculated to count the number of viable bacteria in each culture tube. The bacterial suspension was centrifuged, the culture supernatant was collected, and the supernatant was added to the cultured macrophages to measure its effect on macrophage inflammatory factors such as tumor necrosis factor α (TNFα), nitric oxide (NO) Inducing ability; At the same time, the morphology of bacteria in each culture tube was observed with optical microscope. Results: Shigella dysenteriae cultured in nutrient broth could rapidly dissolve the bacteria under the action of CFT (16 × MIC, 32 × MIC), accompanied by the release of a large amount of inflammatory active substances, the culture supernatant was cultured in vitro macrophages Cells can induce a large number of TNFα, NO production; L ZM combined with CFT both to prevent bacterial lysis, but also to maintain the same with CFT sterilization alone; microscopic observation, the death of bacteria adhesion to each other to form aggregates; After treatment can reduce macrophage activation, reduce TNFα, NO production. In blood culture system, CFT (16 × MIC, 32 × MIC) had strong bactericidal effect on Shigella dysenteriae, but was weaker than that in broth culture system. Compared with CFT (16 × MIC, 32 × MIC) single drug treatment has a stronger bactericidal effect, and can significantly reduce the release of NO. Conclusions: The combination of L ZM and CFT prevents the dissolution of Shigella dysenteriae and reduces the release of bacterial proinflammatory substances (mainly endotoxins)