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目的:检测IQ结构域三磷酸鸟苷合酶-激活蛋白1(IQ domain GTPase-activating protein 1,IQGAP1)在哈萨克族食管癌组织中的表达,分析其与临床病理参数的相关性。方法:采用逆转录PCR(reverse transcription polymerase chain reaction,RTPCR)检测48例哈萨克族食管癌切除标本中癌组织与远端无癌组织中IQGAP1 mRNA表达水平,并分析其与临床病理指标的关系。结果:癌组织中IQGAP1 mRNA表达阳性率(50%)高于其远端无癌组织(21%),其差异具有统计学意义(P<0.05);但IQGAP1 mRNA的表达与癌组织分化程度、病理分期及有无淋巴结转移均无相关性(P>0.05)。结论:IQGAP1基因的表达可能与哈萨克族食管癌的发生有关。
AIM: To detect the expression of IQ domain GTPase-activating protein 1 (IQGAP1) in Kazakh esophageal cancer tissue and analyze its correlation with clinicopathological parameters. Methods: Reverse transcription polymerase chain reaction (RTPCR) was used to detect the expression level of IQGAP1 mRNA in 48 cases of esophageal carcinoma of Kazakh esophageal cancer and distal non-cancerous tissue. The relationship between IQGAP1 mRNA and clinicopathological parameters was analyzed. Results: The positive rate of IQGAP1 mRNA (50%) in cancer tissues was higher than that in distal non-cancerous tissues (21%) (P <0.05). However, the expression of IQGAP1 mRNA correlated with the degree of differentiation, There was no correlation between pathological stage and lymph node metastasis (P> 0.05). Conclusion: The expression of IQGAP1 gene may be related to the occurrence of Kazakh esophageal cancer.