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在对 10 5份湖北栽培大麦酯酶同工酶研究的基础上 ,采用 RAPD技术对分属于 8种酶谱类型的 10份湖北栽培大麦品种进行了研究 .6 3个随机引物中有 12个引物产生稳定多态性的带 ,共扩增出 73条带 ,其中 46条表现出多态性 .将每一条带视为一个独立的性状 ,按其有无列出二元数据矩阵 ,计算 Nei氏相似性系数 (S)和遗传距离 (D) ,列出遗传距离矩阵 ,采用 PHYL IP3.5 c软件包进行聚类 .讨论了 RAPD的稳定性以及 RAPD标记与同工酶标记的异同 .
Based on the study of 105 esterase isoenzymes in barley cultivated in Hubei Province, 10 Hubei cultivated barley cultivars belonging to 8 zymogram types were studied by RAPD technique. Of the 6 random primers, 12 primers A total of 73 bands were amplified and 46 of them showed polymorphism, each band was regarded as an independent trait, according to the presence or absence of a binary data matrix to calculate Nei’s Similarity coefficient (S) and genetic distance (D), the genetic distance matrix is listed and the PHYL IP3.5 c software package is used for clustering.The stability of RAPD and the similarities and differences between RAPD markers and isozyme markers are discussed.