石斛是名贵药材,其中铁皮石斛(Dendro-bium officinale)的药用价值最高。它分布于秦岭、伏牛山、大别山及其以南山地,因过度采掘,已濒临绝迹。石斛种子细小,一个蒴果内所含种子达数万粒之多。但其种子成熟期长达6个月之久,且在自然条件下萌发率非常低。为了援救此濒危植物,我们用铁皮石斛的种子进行组培,已获得了大量试管苗。促使种子萌发及分化用的培养基为Knudson+NAA 0.5毫克/升;促使幼苗快速生长用MS+NAA 0.5毫克/升。两种培养基均用12％的香蕉汁或豆芽 Dendrobium is a valuable medicinal material, of which Dendro-bium officinale has the highest medicinal value. It is located in the Qinling Mountains, Funiu Mountain, Dabie Mountain and its southern mountains, due to excessive mining, has been on the verge of extinction. Dendrobium seed small, a capsule containing seeds up to tens of thousands. But its seed maturity is as long as 6 months, and its germination rate is very low under natural conditions. In order to rescue this endangered plant, we used the seeds of Dendrobium candidum for tissue culture, and obtained a large number of test-tube seedlings. Promote seed germination and differentiation medium for Knudson + NAA 0.5 mg / l; to promote the rapid growth of seedlings with MS + NAA 0.5 mg / l. Both media use 12% banana juice or bean sprouts