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呼吸代谢水平是鱼类生理学的一个重要指标,建立准确的测量方法对于研究鱼类生理学具有重要意义。本文采用在不同温度条件下对鱼类呼吸代谢研究中常用的封闭流水式和开放静水式测量方法进行了比较研究。结果表明,开放静水条件和封闭流水条件均可以有效测量不同温度条件下的耗氧率,在温度较低时,测量鱼的耗氧率较低,两种方法测得的结果差异较小,结果基本一致;当温度升高,鱼的代谢水平也随之升高,测量结果显示出一定的差异,主要表现为流水封闭条件下测得的耗氧率较高,且耗氧率的急剧上升,在2 8°时出现一个明显的峰值;开放静水条件则耗氧率上升缓慢,且无明显的峰值,而且结果明显低于流水封闭式。其差异可能是由于开放静水条件下,随着水体中氧气的消耗,空气中的氧气与水体中的氧气压力差变大,空气中的氧气通过界面的溶解作用进入水体的量较大导致的。因此,采用静水开放式呼吸测量装置测定鱼类的呼吸代谢水平最好与其他较为准确的方法进行平行试验,了解其中的误差值方可,相对而言,封闭式的测量方法测得的结果更为准确。
The level of respiratory metabolism is an important indicator of fish physiology. Establishing accurate measurement methods is of great significance for the study of fish physiology. In this paper, a comparative study of closed flow and open hydrostatic measurements commonly used in the study of respiratory metabolism in fish under different temperature conditions was carried out. The results showed that both open and closed water conditions could effectively measure the oxygen consumption rate under different temperature conditions. When the temperature was lower, the oxygen consumption rate of fish was lower, and the differences between the two methods were insignificant. Results Basically the same; when the temperature rises, the fish’s metabolic level also rises, the measurement results show some differences, mainly manifested as the higher oxygen consumption rate measured under confined flow conditions, and the oxygen consumption rate rose sharply, A significant peak appeared at 28 ° C; the open oxygen consumption rate increased slowly and showed no obvious peak value, and the result was obviously lower than that of the flowing water closed type. The difference may be due to the open hydrostatic conditions, with the consumption of oxygen in the water, the oxygen in the air and oxygen in the water pressure difference becomes larger, the oxygen in the air through the dissolution of the interface into a larger amount of water. Therefore, the use of open-air open-air respiration measuring apparatus to measure the respiratory metabolism of fish is best carried out in parallel with other more accurate method to understand the error value before it can, relatively speaking, closed-end measurement of the measured results more Accurate.