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利用Ｆｎｙ＿ＣＭＶ株系ＲＮＡ３ｃＤＮＡ克隆，构建了含有全长和编码区缺失５０１个核苷酸的运动蛋白（ＭＰ）基因植物表达载体ｐＢＭＰＲ和ｐＢＭＰＫ。在土壤农杆菌（Ａｇｒｏｂａｃｔｅｒｉｕｍｔｕｍｅｆａｃｉｅｎｓ（ＳｍｉｔｈｅｔＴｏｗｎｓｅｎｄ）Ｃｏｎｎ）ＬＢＡ４４０４介导下转化烟草（ＮｉｃｏｔｉａｎａｔａｂａｃｕｍＬ．）品种“ＮＣ８９”，分别经Ｓｏｕｔｈｅｒｎｂｌｏｔｉｎｇ、ＲＴ＿ＰＣＲ或Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ分析，外源基因已整合到再生植株中并得到表达。抗病性分析表明，含有缺失型ＭＰ基因的Ｒ０代转基因植株抗性较好，接种５０ｄ后，１０株转化植株中仍有５株不表现症状。在自然发病条件下，这５个含有缺失型ＭＰ基因转基因株系在Ｒ１代都表现了一定的抗病性。抗性主要表现为症状出现推迟，严重度减轻。利用ＰＣＲ筛选、种子卡那霉素抗性试验和温室抗病性测定等方法，初步认为Ｒ２代转基因烟草Ｋ＿６＿５株系为转基因抗病纯合系。而含有全长ＭＰ基因的Ｒ０代转化植株，前期没有表现明显的抗病性，但在接种４０ｄ后部分发病植株有恢复健康的趋势。
Using the Fny_CMV RNA3 cDNA clone, we constructed the plant expression vectors pBMPR and pBMPK containing the 501-nucleotide-deleted motor protein (MP) gene. Transformation of Nicotiana tabacum L. NC89 by Agrobacterium tumefaciens (Smithet Townsend) Conn LBA4404 was carried out by Southern blotting, RT-PCR or Western blotting respectively. The exogenous gene was integrated into the regenerated plant and expressed. Disease resistance analysis showed that the R0 transgenic plants containing the deletion type MP gene had better resistance. After 50 days of inoculation, 5 out of 10 transformed plants showed no symptoms. Under natural conditions, the five transgenic lines containing the deleted MP gene showed some disease resistance in R1 generation. Resistance mainly for the symptoms appear delayed, reduce the severity. Using PCR screening, kanamycin resistance test of seed and determination of greenhouse disease resistance, it was initially considered that the R2 generation transgenic tobacco K_6_5 strain was transgenic and resistant homozygous line. However, the R0 transgenic plants that contained the full-length MP gene had no significant disease resistance in the early stage, but some of the plants that had been inoculated 40 days after inoculation tended to recover.