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【目的】探讨Ca(NO3)2胁迫下γ-氨基丁酸(GABA)的浓度效应,为科学合理地应用GABA、提高其综合效益提供理论依据。【方法】选择盐敏感的甜瓜品种“一品天下208”为试验材料,采用1/2山崎甜瓜营养液水培法,设营养液内含80mmol/L Ca(NO3)2胁迫条件下每天叶面喷施0,10,25,50和75mmol/L GABA及胁迫前48h预先叶面喷施25和50mmol/L GABA 7个处理,以营养液栽培为对照(CK),比较各处理对甜瓜幼苗生长和生理指标的影响。【结果】Ca(NO3)2胁迫下,甜瓜叶片光合色素、脯氨酸(Pro)和丙二醛(MDA)含量显著升高,幼苗叶面积、干鲜质量、根体积、根表面积、总根长、根尖数和可溶性蛋白(Pr)含量显著降低,甜瓜幼苗的生长受到显著抑制。Ca(NO3)2胁迫下,甜瓜叶片光合色素、Pro和MDA含量随着外源GABA浓度的增加先降低后升高,并且在50mmol/L时达到最小值;Pr含量随外源喷施GABA浓度的增加而先升高后降低,在50mmol/L时达到最大值;外源喷施50mmol/L GABA处理与单纯Ca(NO3)2处理相比,可明显增加幼苗叶面积、干鲜质量、根体积、根表面积、总根长和根尖数,缓解Ca(NO3)2胁迫对甜瓜幼苗生长的抑制。另外,相同GABA浓度相比,Ca(NO3)2胁迫后喷施较胁迫前预喷施效果好。【结论】叶面喷施50mmol/L GABA有利于提高幼苗对Ca(NO3)2盐胁迫逆境的适应能力。
【Objective】 The purpose of this study was to investigate the concentration effect of γ-aminobutyric acid (GABA) under Ca (NO 3) 2 stress and to provide a theoretical basis for the scientific and rational application of GABA and its comprehensive benefits. 【Method】 The salt-sensitive melon variety “Yipintianxia208 ” was selected as test material, and 1/2 Yamasaki muskmelon nutrient solution hydroponics was used. The nutrient solution containing 80mmol / L Ca (NO3) Spraying 0, 10, 25, 50 and 75 mmol / L GABA on the surface and pre-spraying 25 and 50 mmol / L GABA on the foliage 48 hours before stress, respectively, Growth and physiological indicators. 【Result】 The results showed that under Ca (NO3) 2 stress, photosynthetic pigments, proline (Pro) and malondialdehyde (MDA) contents of melon leaves were significantly increased, leaf area, dry and fresh weight, root volume, Length, apical number and soluble protein (Pr) content decreased significantly, the growth of muskmelon seedlings was significantly inhibited. Under Ca (NO3) 2 stress, the contents of photosynthetic pigments, Pro and MDA in melon leaves firstly decreased and then increased with the increase of exogenous GABA concentration, and reached the minimum value at 50 mmol / L. Pr content increased with the GABA concentration , And reached its maximum at 50 mmol / L. Compared with Ca (NO3) 2 treated with 50 mmol / L GABA, the leaf area, dry and fresh weight, Volume, root surface area, total root length and root tips, alleviate the inhibition of Ca (NO3) 2 stress on the growth of melon seedlings. In addition, compared with the same GABA concentration, Ca (NO3) 2 stress spray better than the pre-stress effect. 【Conclusion】 Foliar application of 50 mmol / L GABA was beneficial to improve seedling adaptability to Ca (NO3) 2 stress.