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Surface charge characteristics of a membrane can be determined by the streaming potential. In order to obtain more accurate streaming potential value during the measurement, four measurement operation modes were investigated in this study, and among the four modes, the steady mode with pressure stepped downward was considered the best one. Experimental results showed that the effects of compaction on the streaming potential measurement for a microfiltration membrane was more obvious than that for a ultrafiltration membrane. Both feed pH and presoaking could affect the measurement of streaming potential.