In order to explore the differences of amino acids in root exudates and plant tissues,we selected four flue-cured tobacco varieties [Yunyan 87(Ⅰ),K326( Ⅱ),NC102( Ⅲ),Hongda( Ⅳ) ]as the test materials and studied the components and mass fractions of amino acids in rhizosphere soil,non rhizosphere soil,roots and leaves via high performance capillary zone electrophoresis. Ten amino acids were detected out from four varieties,including histidine,lysine,tryptophan,phenylalanine,valine,threonine,proline,serine,alanine and glycine. The mass fractions of amino acids in leaves and roots were higher than those in rhizosphere soil and non rhizosphere soil. In rhizosphere soil and non-rhizosphere soil,the mass fractions of lysine,phenylalanine,threonine,proline and glycine in susceptible varieties( Ⅲ,Ⅳ) were higher than those in resistant varieties(Ⅰ,Ⅱ). In rhizosphere soil,the total amino acids successively were Ⅳ > Ⅲ >Ⅰ> Ⅱ,and in non rhizosphere soil were Ⅲ > Ⅳ >Ⅰ> Ⅱ. The total amino acids in roots of variety Ⅳ was the highest,up to 6 359. 195 μg / g. In leaves,the total amino acids in varietiesⅠand Ⅱ were higher than those in varieties Ⅲ and Ⅳ. The results indicated that amino acids could secrete to rhizosphere via root secretion,and the compositions and mass fractions of amino acids varied with flue-cured tobacco varieties,thus the secretion amount of susceptible varieties was higher than that of resistant varieties. In order to explore the differences of amino acids in root exudates and plant tissues, we selected four flue-cured tobacco varieties [Yunyan 87 (Ⅰ), K326 (Ⅱ), NC102 (Ⅲ), Hongda (Ⅳ)] as the test materials and studied the components and mass fractions of amino acids in rhizosphere soil, non rhizosphere soil, roots and leaves via high performance capillary zone electrophoresis. Ten amino acids were detected out from four varieties, including histidine, lysine, tryptophan, phenylalanine, valine, threonine , proline, serine, alanine and glycine. The mass fractions of amino acids in leaves and roots were higher than those in rhizosphere soil and non rhizosphere soil. The mass fractions of lysine, phenylalanine, threonine, proline and glycine in susceptible varieties (Ⅲ, Ⅳ) were higher than those in resistant varieties (Ⅰ, Ⅱ). In rhizosphere soil, the total amino acids succeeded Ⅳ> Ⅲ> Ⅰ> Ⅱ, and in non rhizosphere soil were Ⅲ> Ⅳ> Ⅰ> . The total amino acids in roots of variety IV was the highest, up to 6 359. 195 μg / g. In leaves, the total amino acids in varieties I and II were higher than those in varieties Ⅲ and Ⅳ. The results indicated that amino acids could secrete to rhizosphere via root secretion, and the compositions and mass fractions of amino acids varied with flue-cured tobacco varieties, thus the secretion amount of susceptible varieties was higher than that of resistant varieties.