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目的通过对OXA、AdeABC、CarO基因及生物膜在临床分离的亚胺培南耐药和敏感鲍曼不动杆菌(Acinetobacter Baumannii,AB)中分布特征的研究,进一步探讨AB对亚胺培南的耐药机制。方法运用PCR技术对OXA、AdeABC、CarO基因进行检测;采用微量滴定板法制备生物膜模型及定量实验。结果 OXA-23在亚胺培南耐药和敏感菌株中的检出率分别为99.1%和2.9%(P<0.05);OXA-51基因在所有检测菌株中均被检出。Ade ABC在耐药菌株中的检出率为98.1%,而在敏感菌株中的检出率仅为64.7%(P<0.05)。CarO基因在耐药和敏感菌株中的检出率分别为97.2%和99.0%,P>0.05。产生物膜菌株在耐药和敏感菌株分别为67.9%和75.5%,P>0.05。结论 OXA-51基因是确认是否为AB的标志性基因;OXA-23基因在AB对碳青霉烯类抗生素耐药机制中起到了重要的作用;Ade ABC外排泵在降低AB对亚胺培南的敏感性,增强细菌耐药性方面起着重要作用;外膜蛋白CarO的改变对于降低AB对亚胺培南的敏感性的可能性不大;临床分离的AB所引发的感染多数与生物膜的形成有关。
OBJECTIVE: To investigate the distribution characteristics of OXA, AdeABC, CarO gene and biofilm in clinically isolated imipenem-resistant and sensitive Acinetobacter Baumannii (AB) Resistance mechanism. Methods The OXA, AdeABC and CarO genes were detected by PCR. The biofilm model and quantitative experiment were prepared by microtiter plate method. Results The detection rates of OXA-23 in resistant and susceptible strains of imipenem were 99.1% and 2.9%, respectively (P <0.05). The OXA-51 gene was detected in all tested strains. The detection rate of Ade ABC in drug-resistant strains was 98.1%, while the detection rate in sensitive strains was only 64.7% (P <0.05). The detection rates of CarO gene in resistant and susceptible strains were 97.2% and 99.0% respectively, P> 0.05. The biofilm strains were resistant and susceptible strains were 67.9% and 75.5% respectively, P> 0.05. Conclusion OXA-51 gene is a marker gene to confirm whether it is AB. OXA-23 gene plays an important role in the mechanism of resistance to carbapenem in AB. South sensitivity and enhance bacterial resistance plays an important role; changes in the outer membrane protein CarO is not likely to reduce the sensitivity of AB to imipenem; clinical isolation of AB-induced infections and biological Membrane formation related.