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目的:阐明酒精性股骨头坏死及补肾中药对其治疗作用的机制。方法:以浓度0.3、0.9、1.5mol/L的酒精对OS-732成骨样细胞进行干预,通过流式细胞仪等观察其坏死和凋亡的情况;选择浓度为0.15mol/L酒精干预后的细胞模型用六味地黄丸含药血清治疗,观察其治疗作用。结果:成骨样细胞凋亡率和坏死的程度与干预酒精的浓度及干预时间正相关,其中多形状的细胞变成圆形,细胞突起短缩变细,核糖体、内质网减少,线粒体部分皱褶消失。经六味地黄丸治疗后的细胞突起恢复,内质网、线粒体数量有所增加,结构部分恢复。结论:酒精可引起成骨样细胞死亡和凋亡,且与酒精浓度及干预时间成正相关,六味地黄丸对恢复细胞的活性有一定作用。
Objective: To elucidate the mechanism of alcoholic femoral head necrosis and its therapeutic effect. METHODS: OS-732 osteoblast-like cells were treated with alcohol at a concentration of 0.3, 0.9, and 1.5 mol/L. The necrosis and apoptosis were observed by flow cytometry; the alcohol concentration was 0.15 mol/L after intervention. The cell model was treated with Liuwei Dihuang Pill containing serum and its therapeutic effect was observed. RESULTS: The rate of apoptosis and necrosis of osteoblast-like cells was positively correlated with the concentration of alcohol and the intervention time. Multi-shape cells became round, cell protrusions shortened and the ribosomes, endoplasmic reticulum decreased, and mitochondria decreased. Some wrinkles disappear. After treatment with Liu Wei Di Huang Wan, the cell protrusions recovered, the number of endoplasmic reticulum and mitochondria increased, and the structure recovered partially. Conclusion: Alcohol can cause osteoblast-like cell death and apoptosis, and is positively correlated with alcohol concentration and intervention time. Liuwei Dihuang Pill plays a certain role in restoring cell activity.