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近年来,由胶胞炭疽病菌Colletotrichum gloeosporioides引起的梨炭疽病在砀山酥梨产区普遍发生。为探讨生防菌对梨炭疽病的控制作用,采用平板对峙生长法和喷雾法研究了枯草芽胞杆菌sf 628对梨炭疽病的室内外防治效果。枯草芽胞杆菌sf 628发酵菌液、100倍和500倍稀释液对胶胞炭疽病菌菌丝生长的平板抑制率分别为91.45%、88.80%和86.63%。梨果先喷施生防菌液sf628,24 h后接种炭疽病菌,对梨炭疽病的防治效果为83.74%;梨果先接种炭疽病菌,24 h后再喷施生防菌液sf 628,对梨炭疽病的防治效果为56.82%。生防菌sf 628可湿性粉剂100、1000和2000倍稀释液对梨炭疽病的田间防治效果分别为75.89%、60.78%和50.60%,常规对照药剂多菌灵的防治效果为61.10%。研究表明,枯草芽胞杆菌sf 628是一株对梨炭疽病有较好控制作用的生防菌。
In recent years, pear anthracnose caused by colletotrichum gloeosporioides is commonly found in Dangshan pear production area. In order to study the control effect of biocontrol bacteria on pear anthracnose, the indoor and outdoor control effects of Bacillus subtilis sf 628 on pear anthracnose were studied by plate growth and spraying method. Bacillus subtilis sf 628 fermentation broth, 100-fold and 500-fold dilutions of Colletotrichum gloeosporioides mycelial growth inhibition were 91.45%, 88.80% and 86.63%. Pear fruit first spraying antibacterial sf628, 24 h after inoculation of anthrax bacteria, anthracnose prevention and control of pear on the effect of 83.74%; pear first inoculation of anthrax bacteria, and then spraying biocontrol sf 628 24 h, Pear anthracnose prevention and control effect of 56.82%. The control effects of biocontrol sf 628 wettable powder 100, 1000 and 2000 times dilutions on pear anthracnose were 75.89%, 60.78% and 50.60%, respectively. The control efficacy of the conventional control agent carbendazim was 61.10%. Studies have shown that Bacillus subtilis sf 628 is a pest control anthrax has better control of biocontrol bacteria.