论文部分内容阅读
应用SCoT分子标记技术对71份贵州桃种质进行遗传多样性分析,以期从分子水平上揭示其遗传多样性及资源间的遗传关系,为科学保存与利用贵州桃种质资源提供依据。结果表明:(1)16条引物共检测出192个位点,其中多态性位点数156个,多态性比例为81.25%,平均每条引物扩增位点为12个;供试材料Nei’s遗传多样性指数(H)为0.265 0±0.186 1,Shannon’s信息指数(I)为0.400 3±0.254 3,遗传相似系数变幅为0.400 0~0.852 5。(2)UPGMA聚类分析显示,在相似系数0.65处可将71份桃资源分成6类,其中2份白花桃资源、2份血桃资源、2份青桃资源分别为同名异物。研究认为贵州桃种质具有较丰富的遗传多样性,采用该分子标记技术区分了同名异物的桃资源。
Genetic diversity of 71 Peach germplasms in Guizhou was analyzed using SCoT molecular markers in order to reveal their genetic diversity and their genetic relationship at the molecular level, which provided the basis for scientific preservation and utilization of Guizhou peach germplasm. The results showed that: (1) A total of 192 loci were detected by 16 primers, of which 156 were polymorphic loci with a polymorphism ratio of 81.25%, with an average of 12 loci per primer. Nei’s The genetic diversity index (H) was 0.265 0 ± 0.186 1, Shannon’s information index (I) was 0.400 3 ± 0.254 3, and genetic similarity coefficient was 0.400 0 ~ 0.852 5. (2) UPGMA cluster analysis showed that 71 peach cultivars could be divided into 6 categories at the similarity coefficient of 0.65, of which 2 were Baishuao, 2 peach and 2 peach, respectively. The study found that Guizhou peach germplasm has a rich genetic diversity, using the molecular marker technology to distinguish the same name foreign material peach resources.