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目的通过比较非选择性β受体阻滞剂普萘洛尔和选择性β_1受体阻滞剂美托洛尔对小鼠血管瘤(EOMA细胞)细胞体外增殖及凋亡的调控作用,初步探讨普萘洛尔对小鼠血管瘤的治疗作用及可能机制,为临床应用β受体阻滞剂治疗婴幼儿血管瘤提供参考。方法体外培养EOMA细胞,取对数生长期EOMA细胞,随机分为药物组和对照组,用不同浓度普萘洛尔和关托洛尔作用:EOMA细胞,分别干预24h。通过连续光谱多功能酶标仪在570nm和630nm波长处测定以上质量浓度下光吸收值。应用四甲基偶氮唑盐比色法(MTT)检测细胞存活率、吖啶橙染色检测细胞凋亡情况,观察普萘洛尔和美托洛尔对EOMA细胞体外增殖和凋亡的影响。结果随着两种药物浓度逐渐升高,细胞存活率逐渐下降;两种药物在相同浓度时对EOMA细胞存活率的影响差异具有显著性,普萘洛尔组细胞存活率低于关托洛尔组。两种药物作用24h后,EOMA细胞凋亡数量与药物浓度呈正比;在药物浓度≥50μmol/L时,普萘洛尔组EOMA细胞凋亡数量高于美托洛尔组,差异有显著性(P<0.05)。美托洛尔作用24h后,不同浓度组对EOMA细胞体外增殖的抑制作用明显低于相同浓度普萘洛尔,无明显诱导细胞凋亡作用(P>0.05)。结论与非选择性β肾上腺素受体阻滞剂普萘洛尔相比,选择性β_1肾上腺素受体阻滞药物关托洛尔并不能有效抑制小鼠血管瘤内皮细胞(EOMA细胞)体外增殖及诱导其凋亡。β肾上腺素受体阻滞剂类药物治疗血管瘤的具体机制尚有待进一步的研究。
Objective To compare the effects of propranolol, a selective non-selective β-blocker, and metoprolol, a selective β 1 blocker, on the proliferation and apoptosis of mouse hemangiomas (EOMA cells) in vitro. Propranolol treatment of hemangiomas in mice and possible mechanisms for the clinical application of β-blockers for the treatment of infantile hemangiomas to provide a reference. Methods Eoma cells were cultured in vitro and logarithmic growth phase EOMA cells were randomly divided into drug group and control group. ENOS cells were treated with different concentrations of propranolol and tetonolol for 24 h. The absorbance value at the above mass concentration was measured at a wavelength of 570 nm and 630 nm by a continuous spectrum multi-functional microplate reader. MTT assay was used to detect the cell viability, and acridine orange staining was used to detect the cell apoptosis. The effects of propranolol and metoprolol on proliferation and apoptosis of EOMA cells were observed. Results As the concentration of the two drugs gradually increased, cell survival rate gradually decreased; the effect of the two drugs on the survival rate of EOMA cells at the same concentration was significant, and the survival rate of propranolol group was lower than that of guantalol group. After 24 h of treatment with the two drugs, the number of apoptosis in EOMA cells was positively correlated with the drug concentration; when the drug concentration was ≥50 μmol/L, the number of apoptosis in the EOMA cells in the propranolol group was higher than that in the metoprolol group (the difference was significant). P<0.05). After 24h of metoprolol treatment, the inhibitory effects of different concentrations on the proliferation of EOMA cells in vitro were significantly lower than those of the same concentration of propranolol, and no significant apoptosis was induced (P>0.05). CONCLUSION: Selective β 1 adrenergic blocker guantalol is not effective in inhibiting the proliferation of mouse hemangioendothelial cells (EOMA cells) in vitro compared with propranolol, a nonselective β-adrenoceptor blocker. And induce its apoptosis. The specific mechanism of β-adrenoceptor blocker drugs for hemangiomas remains to be further studied.