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利用Affymetrix水稻表达谱芯片(GeneChip Rice Genome Array)研究抗旱杂草稻HEB07-2与巴西陆稻(IAPAR9)在PEG模拟干旱胁迫下根系基因表达变化。结果表明,杂草稻HEB07-2转录组对干旱信号的响应程度与方向均与巴西陆稻存在很大差异,HEB07-2以正向调控为主,而巴西陆稻以负调控为主。干旱胁迫下杂草稻HEB07-2与巴西陆稻分别有6878个和2923个基因表达,其中HEB07-2受干旱胁迫诱导上调的基因有4693个,下调的基因有2185个;而巴西陆稻则分别有983个和1940个。在差异基因表达的倍数上也是HEB07-2高于巴西陆稻。进一步的GO分析表明,在干旱胁迫下,HEB07-2和IAPAR9根系基因响应途径的差异表现为HEB07-2在钾离子转运(GO:0006813)、次生物质代谢(GO:0019748)、细胞生长(GO:0016049)、葡萄糖代谢(GO:0006006)、跨膜离子转运器活性(GO:0015075)、亚铁血红素结合体(GO:0020037)、氧化还原酶活性(GO:0016491)等方面基因显著上调,这与生理数据和表型数据一致。
Using GeneChip Rice Genome Array, Affymetrix rice gene expression profiles of drought-tolerant weedy rice (HEB07-2) and Brazilian upland rice (IAPAR9) under PEG simulated drought stress were studied. The results showed that both the response and drought response of weedy rice HEB07-2 transcriptome were different from those of Brazilian landrace. HEB07-2 was predominantly positive, while that of Brazilian Upland rice was negatively regulated. Under drought stress, 6878 and 2923 genes were expressed in weedy rice (HEB07-2) and inbred landrace in Brazil respectively. Among them, 4,693 genes were upregulated in HEB07-2 and 2,185 genes were downregulated in drought stress, while those in brazilian upland rice There are 983 and 1940 respectively. HEB07-2 was also higher than that of Brazilian Upland rice at multiples of differential gene expression. Further GO analysis showed that the response of HEB07-2 and IAPAR9 roots under drought stress showed that the response of HEB07-2 to potassium ion transport (GO: 0006813), metabolism of secondary metabolites (GO: 0019748), cell growth ( GO: 0016049), glucose metabolism (GO: 0006006), transmembrane ion transporter activity (GO: 0015075), heme conjugate (GO: 0020037), oxidoreductase activity Up, which is consistent with the physiological and phenotypic data.