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目的探讨重庆地区人群血管内皮素-1(endothelin-1,ET-1)基因Lys198Asn、TaqⅠ酶切位点基因多态性与原发性高血压(essential hypertension,EH)的关系。方法运用序列特异引物聚合酶链反应(sequence specific primer poly-merase chain reaction,SSP-PCR)和限制性内切酶片段长度多态性(restriction fragment length polymorphism,RFLP)方法分析86例EH患者和138例非EH患者的Lys198Asn和TaqⅠ2个位点基因多态性。结果体质量指数(body mass index,BMI)<25 kg/m2人群中高血压组的TaqⅠ酶切位点基因型分布频率为:GG型26例(72.2%),GA+AA型10例(27.8%);对照组:GG型28例(50.0%),GA+AA型28例(50.0%)。Logistic回归分析显示BMI<25 kg/m2人群中携带GG基因型(TaqⅠ酶切位点)的患者发生高血压的风险增加(P=0.037,OR=2.597)。线性回归结果显示收缩压(SBP)在BMI<25 kg/m2人群的GG型平均值为145.06,显著高于GA+AA型平均值131.97(P=0.018)。结论在BMI<25 kg/m2人群中TaqⅠ酶切位点GG基因型更易导致SBP升高,GG基因型可能是BMI较小人群血压升高的一个危险因素。
Objective To investigate the relationship between polymorphisms of Lys198Asn, Taq Ⅰ gene and essential hypertension (EH) in endometriosis-1 (ET-1) gene in Chongqing population. Methods Totally 86 patients with EH and 138 patients with EH were analyzed by sequence specific primer poly-merase chain reaction (SSP-PCR) and restriction fragment length polymorphism (RFLP) Cases of non-EH patients Lys198Asn and Taq Ⅰ two locus gene polymorphisms. Results The distribution frequencies of Taq Ⅰ restriction sites in hypertension group with body mass index (BMI) <25 kg / m2 were 26 cases (72.2%) of GG type and 10 cases (27.8% ); Control group: GG type in 28 cases (50.0%), GA + AA type in 28 cases (50.0%). Logistic regression analysis showed an increased risk of developing hypertension in patients with GG genotypes (Taq I restriction sites) in BMI <25 kg / m2 (P = 0.037, OR = 2.597). The linear regression results showed that SBP was 145.06 for GG type in BMI <25 kg / m2 and significantly higher than 131.97 for GA + AA type (P = 0.018). Conclusions The GG genotype at Taq Ⅰ restriction site is more likely to cause an increase in SBP in BMI <25 kg / m 2 population, and GG genotype may be a risk factor for hypertension in a small population of BMI.