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目的观察雄激素受体(AR)共激活物DDC在氟他胺对前列腺癌雄激素敏感细胞系LNCaP的作用的表达变化。方法噻唑蓝比色法(MTT),流式细胞术等技术,研究氟他胺对LNCaP细胞的作用,并使用基因芯片对其DDC在其中的作用作了初步探讨。结果LNCaP细胞在7d内受到氟他胺的刺激而加速生长,但5代后细胞增殖受到抑制。此种效应与剂量相关。细胞增殖主要被阻断于G1/S期。其相关机制主要是AR共激活物DDC的表达受到抑制。结论氟他胺对LNCaP的作用为双向,即在低浓度和较短作用时间内为增殖,在高浓度和较长作用时间后为抑制。此种抑制效应可能是通过抑制DDC的表达,影响下游细胞周期相关基因的表达,从而阻断细胞周期于G1/S调控点。
Objective To observe the effect of androgen receptor (AR) co-activator DDC on the expression of flutamide on the prostate cancer androgen sensitive cell line LNCaP. MethodsThiazolyl blue colorimetry (MTT), flow cytometry and other techniques to study the effect of flutamide on LNCaP cells, and the use of gene chip DDC in which the role of a preliminary study. Results LNCaP cells were stimulated by flutamide within 7 days, however, cell proliferation was inhibited after 5 passages. This effect is dose related. Cell proliferation was mainly blocked in the G1 / S phase. Its related mechanism is mainly that the expression of DD co-activator DDC is inhibited. Conclusion The effect of flutamide on LNCaP is bi-directional, that is, proliferation at low concentration and short duration of action, and inhibition at high concentrations and longer duration of action. This inhibitory effect may be through the inhibition of DDC expression, affecting the expression of downstream cell cycle related genes, thus blocking the cell cycle at the G1 / S regulatory point.