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目的 为探讨压力波的致聋机理 ,对豚鼠耳蜗血管纹 (SV)、毛细胞 (HC)中心钠素免疫活性 (ANP -IR)的改变及与听阈阈移的相关性进行研究。方法 采用免疫细胞化学 (ABC法 )法、图像分析听性脑干反应测听技术 (ABR)对压力波暴露后不同时间分组的豚鼠耳蜗SV、HC中ANP -IR产物进行检测。结果 压力波暴露后 6、12、2 4h和 48h组SV组织中ANP -IR光密度值较对照组均有明显的差异 (P <0 0 5 ) ,其中 2 4h组最高 ;冲击波暴露后 6、12、2 4h组内毛细胞 (IHC)中ANP -IR阳性产物的光密度值较对照组明显增高 (P <0 0 5 ) ,其中以 12h组最高。二者的变化均与听阈阈移有明显的正相关性 (r1 =0 .8175 ,P >0 .0 5 ;r2 =0 .9185 ,P >0 .0 5 )。而外毛细胞 (OHC)中ANP -IR阳性产物变化不明显。结论 压力波暴露后 ,SV组织中ANP的增高可能是内耳的一种代偿机制 ;IHC和OHC中ANP -IR的变化可能是冲击波对其损伤机制的不同表现
Objective To investigate the mechanism of deafness caused by pressure wave, and to study the changes of atrial natriuretic peptide immunoreactivity (ANP-IR) in guinea pig cochlear stria vascularis (SV) and hair cells (HC) and its correlation with threshold shift. Methods The ANP-IR products in SV and HC of cochlear of guinea pigs were detected by immunocytochemistry (ABC method) and auditory brainstem response audiometry (ABR) after exposure to pressure wave. Results Compared with the control group, the ANP-IR optical density values of SV in 6, 12, 24 h and 48 h groups were significantly different after pressure wave exposure (P <0 05), of which 24 h was the highest, The optical density of ANP-IR positive product in hair cells (IHC) group was significantly higher than that in control group at 12 and 24 hours (P <0.05), and was highest in 12h group. Both changes were significantly positive correlation with threshold shift (r1 = 0.8175, P> 0.05; r2 = 0.9185, P> 0.05). The outer hair cells (OHC) ANP-IR-positive products did not change significantly. Conclusions The increase of ANP in SV tissue may be a compensatory mechanism of inner ear after pressure wave exposure. The changes of ANP-IR in IHC and OHC may be different manifestations of shock wave on its injury mechanism